文献类型：期刊文献

中文题名：不同配伍比例下生姜对黄芩中化学成分溶出率的影响

英文题名：Effect of Zingiberis Rhizoma Recens on Dissolution Rate of Chemical Components in Scutellariae Radix Under Different Compatibility Ratios

作者：黄钰芳[1];杨志军[1];邵晶[1];杨秀娟[1,2];曹瑞[1];颜春鲁[2];曹强[1]

第一作者：黄钰芳

机构：[1]甘肃中医药大学药学院,兰州730000;[2]敦煌医学与转化教育部重点实验室,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2020

卷号：37

期号：17

起止页码：2086

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：甘肃省高等学校科研项目(2016B-054);甘肃省中医药管理局中医药科研课题(GZK-2019-20);敦煌医学与转化省部共建教育部重点实验室开放基金项目(DHYX17-08,DHYX18-11)。

语种：中文

中文关键词：黄芩;生姜;相恶配伍;物质基础

外文关键词：Scutellariae Radix;Zingiberis Rhizoma Recens;mutual imhibition;material basis

摘要：目的探讨黄芩配伍不同比例生姜前后黄芩中化学成分溶出率的变化,基于微观量化标准揭示"生姜恶黄芩"的科学内涵,为临床应用提供一定的科学参考。方法采用Agilent C18色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇和0.1%磷酸梯度洗脱,柱温30℃,进样量20μL,流速1 mL·min-1,检测波长280 nm。建立黄芩-生姜不同比例提取液的HPLC色谱图,以黄芩单品图谱为对照,标定共有峰,分析不同配伍比例下各图谱的变化,考察黄芩特征峰相对峰面积与配伍比例的关系,并分析黄芩中4种指标性成分的含量变化。结果黄芩单品提取液分离得到30个色谱峰,其中15,19,24和29号峰分别为黄芩苷、汉黄芩苷、黄芩素和汉黄芩素;不同配伍比例中共有峰为22个,与单品图谱相比较,其余配伍比例中均存在黄芩特征峰消失现象,且不同比例下消失峰的种类和数目存在差异;与黄芩单品相比,各比例中除个别色谱峰外黄芩特征峰相对峰面积总体上呈不同程度的下降,其中黄芩-生姜1∶1中下降程度最小,且此比例下除黄芩苷外,汉黄芩苷、黄芩素及汉黄芩素的含量较单品下降程度均不明显,而在其余比例中4成分的含量下降程度均显著(P<0.05)。结论黄芩配伍不同比例生姜后,总体降低了黄芩中化学成分的溶出率,但不是所有成分的溶出率均降低;黄芩-生姜1∶1配伍对黄芩溶出率的影响最小。
OBJECTIVE To reveal the scientific connotation of the compatibility of Zingiberis Rhizoma Recens and Scutellariae Radix based on the microscopic quantitative standard, and provide some scientific references for clinical application, the change of chemical components dissolution rate of Scutellariae Radix was explored before and after different proportion of Scutellariae Radix was mixed with Zingiberis Rhizoma Recens. METHODS The separation was performed on Agilent C18 column(4.6 mm×150 mm, 5 μm) with mobile phase composed of methanol-0.1% phosphoric acid solution for gradient elution at the flow rate of 1 mL·min-1, the column temperature was set at 30 ℃, the injection volume was 20 μL, and the detection wavelength was set at 280 nm. HPLC chromatogram of extracts with different proportions of Scutellariae Radix and Zingiberis Rhizoma Recens was established, and the common peaks were demarcated with the single chromatogram of Scutellariae Radix as the control. The changes of each chromatogram under different compatibility ratios were analyzed, and the relationship between the relative peak area of characteristic peaks of Scutellariae Radix and compatibility ratio was investigated, and the content changes of four index components in Scutellariae Radix were analyzed. RESULTS Thirty chromatographic peaks were isolated from single extract of Scutellariae Radix, and the peaks of 15, 19, 24 and 29 corresponded to baicalin, wogonside, baicalein and wogonin respectively. Twenty-two common peaks were marked in the chromatograms under different compatibility ratios. Compared with single Scutellariae Radix, the relative peak area of characteristic peaks of Scutellariae Radix decreased in different degrees in the rest proportion except individual chromatographic peaks, but the decrease degree was the smallest in 1∶1 ratio. In this ratio, the content of wogonin, baicalein and wogonin besides baicalin was not significantly lower than that of single product, while the content of the above four components in the other ratios was significantly lower(P<0.05). CONCLUSION The dissolution rate of chemical components in Scutellariae Radix can be reduced when it is mixed with Zingiberis Rhizoma Recens in different proportions, but not all components are reduced. The compatibility of Scutellariae Radix and Zingiberis Rhizoma Recens 1∶1 has the least influence on the dissolution rate of Scutellariae Radix.