文献类型：期刊文献

英文题名：Pathogenic analyses of fungus strains isolated from medicinal Fritillaria przewalskii Maxim. bulb rot

作者：Wu, Rui[1,2];Chen, Yuan[1];Guo, Fengxia[1];Gao, Yuan[1];Zhou, Yang[1]

第一作者：Wu, Rui;武睿

通信作者：Chen, Y[1]

机构：[1]Gansu Agr Univ, Coll Agron, Coll Life Sci & Technol,Gansu Prov Key Lab Aridla, Gansu Key Lab Crop Genet & Germplasm Enhancement, Lanzhou, Peoples R China;[2]Gansu Univ Chinese Med, Pharm Dept, Dingxi, Peoples R China

第一机构：Gansu Agr Univ, Coll Agron, Coll Life Sci & Technol,Gansu Prov Key Lab Aridla, Gansu Key Lab Crop Genet & Germplasm Enhancement, Lanzhou, Peoples R China

通信机构：[1]corresponding author), Gansu Agr Univ, Coll Agron, Lanzhou 730070, Peoples R China.

年份：2021

卷号：169

期号：1

起止页码：1

外文期刊名：JOURNAL OF PHYTOPATHOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000595770200001)】；

基金：National Natural Science Foundation of China, Grant/Award Number: 31660158 and 31360316; Gansu Key Science & Technology Program of Chinese Medical Industry, Grant/Award Number: GYC12-06; Chief Expert of Traditional Chinese Medicinal Industry in Modern Agricultural Industry System provided by Gansu Provincial Department of Agriculture and Pastoral Affairs, Grant/Award Number: GARS-ZYC-1.

语种：英文

外文关键词：bulb rot pathogens; Fritillaria przewalskii Maxim; isolation and identification; pathogenicity

摘要：Bulb rot causes a great loss of rare perennial medicinal Fritillaria przewalskii Maxim., which is exacerbated with growth year and seriously constrains the plant productivity, but the pathogens responsible for the disease were still unknown. In this paper, we attempted to explore the potential pathogens leading to bulb rotting and their occurrence patterns in artificial domesticated F. przewalskii. Fungus strains were isolated from the bulb rot spot of the 3- to 5-year-old diseased F. przewalskii plants by tissue separation, further assayed for pathogenicity according to Koch's law and finally identified by both morphological and molecular methods. Seven pathogenic strains (F1-F7) were detected in 5-year-old rot bulbs, six (F1-F6) in 4-year-old rot bulbs and four (F1, F2, F5, F6) in 3-year-old rot bulbs. All of the strains were able to infect bulbs by stabbing and some exhibited varying levels of aggressiveness. Relative to the non-stabbing controls, the bulbs stab-inoculated with F5, F2, F7, F4, F1, F6, and F3 showed 76.65%, 75.15%, 71.44%, 40.37%, 39.09%, 36.87%, and 34.93% rot after 8 days, respectively. Phylogenetic analysis revealed that these seven strains were clustered into Bionectria ochroleuca (F1, F3, F4), Fusarium oxysporum (F2, F7), Fusarium tricinctum (F5), and Clonostachys rosea (F6). The two species of Fusarium had the strongest pathogenicity, followed by Bionectria ochroleuca and Clonostachys rosea. Although leading to low bulb rot incidence by stab inoculation, F1 showed the highest isolation rate (48.9%) among all strains. Thus, the edible and medicinal bulbs of F. przewalskii are susceptible to synergetic contamination by these seven pathogens at some point after their third year of growth, which has contributed to the species endangered status, with the two strains of Fusarium being the predominant pathogens. To our knowledge, this is the first report on the seven strains of four fungal species causing F. przewalskii bulb rot in China.