文献类型：期刊文献

中文题名：当归中Z-藁本内酯及正丁烯基苯酞近红外定量模型的建立

英文题名：Establishment of quantitative model on Z-ligustilide and Z-butylidenephthalide in Angelica sinensis by near-infrared diffuse reflectance spectroscopy

作者：顾志荣[1];王耀鹏[1];张亚亚[1];孙宇靖[1];王亚丽[1]

第一作者：顾志荣

机构：[1]甘肃中医学院

第一机构：甘肃中医药大学

年份：2015

卷号：32

期号：3

起止页码：271

中文期刊名：计算机与应用化学

外文期刊名：Computers and Applied Chemistry

收录：CSTPCD

基金：国家自然科学基金资助项目(30960037);甘肃省发改委战略新兴产业和产业技术研究与开发专项项目(2011)

语种：中文

中文关键词：当归;Z-藁本内酯;正丁烯基苯酞;近红外光谱

外文关键词：Angelica sinensis; Z-ligustilide; Z-butylidenephthalide; near-infrared diffuse reflectance spectroscopy

摘要：建立快速测定当归中Z-藁本内酯及正丁烯基苯酞的近红外漫反射光谱定量模型。通过载有积分球漫反射装置的近红外光谱仪获得141批当归药材的近红外光谱,以高效液相色谱测定Z-藁本内酯及正丁烯基苯酞的质量分数,利用TQ Analyst 8.0软件的偏最小二乘法建立定量模型。结果表明,所建Z-藁本内酯模型的相关系数为0.9931,校正均方差为0.4464,预测均方差为0.50341交叉验证相关系数为0.9362,交叉验证均方差为1.0355。所建正丁烯基苯酞模型的相关系数为0.9672,校正均方差为0.5253,预测均方差为0.6462:交叉验证相关系数为0.9146,交叉验证均方差为1.0453。Z-藁本内酯及正丁烯基苯酞的平均预测回收率分别为100.99%及102.14%。研究表明,利用近红外光谱技术快速测定当归中Z-藁本内酯及正丁烯基苯酞的含量是可行的。
Using near-infrared diffuse reflectance spectroscopy（N1R） technology, a quantitative model was established to rapidly determine Z-ligustilide and Z-butylidenephthalide in Angelica sinensis. The NIR spectra of 141 batches of Angelica sinensis were collected by integrated sphere; the contents of Z-ligustilide and Z-butylidenephthalide were determined by RP-HPLC; the PLS（partial least squares） quantitative analysis model was established by TQ Analyst 8.0 software. The results showed that the correlation coefficient （R） of Z-ligusti｜ide and Z-butylidenephthalide of the calibration set reached 0.9931 and 0.9672, RMSEC（the root-mean-square error of calibration） reached 0.4464 and 0.5253, RMSEP（the root-mean-square error of prediction） reached 0.5034 and 0.6462, cross-validation correlation coefficient reached 0.9362 and 0.9146, RMSECV（the root-mean-square error of cross-validatinn） reachod 1.0355 and 1.0453, and mean prediction recoveries reached 100.99 % and 102.14 %, respectively. The established method can be routinely used for the content determination of Z-ligustilide and Z-butylidenephthalide in Angelica sinensis.