文献类型：期刊文献

英文题名：Comparative analysis of the molecular response characteristics in Platycodon grandiflorus irradiated with heavy ion beams and X-rays

作者：Liu, Xiao[1,2];Du, Yan[1,2];Xu, Chaoli[1,3];Wang, Fusheng[4];Li, Xuehu[1,2];Liu, Luxiang[5];Ma, Xiaohui[6];Wang, Yuanmeng[1,6];Ge, Linghui[1,6];Ren, Weibin[1,2];Jin, Ling[6];Zhou, Libin[1,2,6,7]

第一作者：Liu, Xiao

通信作者：Zhou, LB[1];Zhou, LB[2];Jin, L[3];Zhou, LB[3];Zhou, LB[4]

机构：[1]Chinese Acad Sci, Inst Modern Phys, Biomed Ctr, Biophys Grp, Lanzhou 730000, Peoples R China;[2]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[3]Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou 730070, Peoples R China;[4]Dingxi Acad Agr Sci, Dingxi 743000, Peoples R China;[5]Chinese Acad Agr Sci, Inst Crop Sci, Natl Ctr Space Mutagenesis Crop Improvement, Natl Key Facil Crop Gene Resources & Genet Improve, Beijing 100081, Peoples R China;[6]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[7]Kejin Innovat Inst Heavy Ion Beam Biol Ind, Baiyin 730900, Peoples R China

第一机构：Chinese Acad Sci, Inst Modern Phys, Biomed Ctr, Biophys Grp, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Sci, Inst Modern Phys, Biomed Ctr, Biophys Grp, Lanzhou 730000, Peoples R China;[2]corresponding author), Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[3]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[4]corresponding author), Kejin Innovat Inst Heavy Ion Beam Biol Ind, Baiyin 730900, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2023

卷号：38

起止页码：87

外文期刊名：LIFE SCIENCES IN SPACE RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001046879500001)】；

基金：Financial support provided by Crop Varietal Improvement and Insect Pests Control by Nuclear Radiation, Youth Innovation Promotion Association of Chinese Academy of Sciences (Y201974) , National Natural Science Foundation of China (11975285, 12135016) , Ecological Planting and Quality Assurance Project of Daodi Medicinal Materials (China Science and Technology for Traditional Chinese Medicine (2020) 153) , Dingxi Municipal Science and Technology Program (DX2021AN10) , are gratefully acknowledged.

语种：英文

外文关键词：Platycodon grandiflorus; Heavy ion beams; X-rays; Transcriptome; Proteome

摘要：The response of plants to radiation is an essential topic in both space plant cultivation and mutation breeding by radiation. In this study, heavy ion beams (HIB) generated by the ground accelerator and X-rays (XR) were used as models of high linear energy transfer (LET) and low LET radiation to study the molecular response mechanism of Platycodon grandiflorus (P. grandiflorus) seedlings after irradiation. The gene and protein expression profiles of P. grandiflorus after 15 Gy HIB and 20 Gy XR radiation were analyzed by transcriptome and proteome. The results showed that the number of differentially expressed genes (DEGs) induced by HIB radiation was less than that of XR group, but HIB radiation induced more differentially expressed proteins (DEPs). Both HIB and XR radiation activated genes of RNA silencing, double-strand break repair and cell catabolic process. DNA replication and cell cycle related genes were down-regulated. The genes of cell wall and external encapsulating structure were upregulated after HIB radiation. The gene expression of protein folding and glucan biosynthesis increased after XR radiation. Protein enrichment analysis indicated that HIB radiation resulted in differential protein enriched in photosynthesis and secondary metabolite biosynthesis pathways, while XR radiation induced differential protein of glyoxylate and dicarboxylate metabolism and carbon metabolism. After HIB and XR radiation, the genes of antioxidant system and terpenoid and polyketide metabolic pathways presented different expression patterns. HIB radiation led to the enrichment of non-homologous end-joining pathway. The results will contribute to understanding the biological effects of plants under space radiation.