文献类型：期刊文献

中文题名：采用正交实验优化黄芪总黄酮的提取工艺

英文题名：Optimization of extraction technology for total flavonoids from Radix Astragali by orthogonal test

作者：童丹[1,2]

第一作者：童丹

机构：[1]定西师范高等专科学校,甘肃定西743000;[2]甘肃中医药大学,甘肃兰州730000

第一机构：甘肃中医药大学定西校区

年份：2020

卷号：39

期号：5

起止页码：60

中文期刊名：中兽医医药杂志

外文期刊名：Journal of Traditional Chinese Veterinary Medicine

基金：校级项目(TD2016YB01)。

语种：中文

中文关键词：黄芪;总黄酮;单因素试验;正交试验;提取

外文关键词：Radix Astragali;total flavonoids;single factor experiments;orthogonal test;extraction

摘要：优化黄芪总黄酮提取效率。以黄芪药材为原料,通过单因素试验和正交试验对黄芪总黄酮提取料液比、乙醇体积分数、提取温度、提取时间等4个关键工艺参数进行优化,得出最佳工艺参数。结果发现,最佳提取工艺为料液比1∶18,乙醇体积分数70%,提取温度70℃,提取时间90 min。该工艺条件下黄芪总黄酮提取量为2.53 mg/g。表明该优化后提取工艺方法更为合理,可为黄芪总黄酮的提取和工业化生产提供理论参考和技术支持。
To obstain high extract rate of total flavonoids,using Radix Astragali as raw material,the influences of solid-liquid ratio,the volume fraction of ethanol,extraction temperature and extraction time on the extraction effect were discussed by single factor experiments and orthogonal test.The best combination of process parameters was obtained:the solid-liquid ratio was 1:18,the volume fraction of ethanol was 70%,the extraction temperature was 70℃,the extraction time was 90 min.Under this process,the yield of total flavonoids was 2.53 mg·g^-1.The results showed that the extraction process was reasonale.It can provide theoretical reference and technical support for the extraction of total flavonoids from Radix Astragali and industrial production.