文献类型：期刊文献

英文题名：Chemical constituents in different parts of seven species of Aconitum based on UHPLC-Q-TOF/MS

作者：Chen, Ling-li[1];Lai, Chang-jiang-sheng[1];Mao, Liu-ying[1];Yin, Bi-wei[1,2];Tian, Mei[1];Jin, Bao-long[1];Wei, Xu-ya[1];Chen, Jin-long[1];Ge, Hui[3];Zhao, Xin[3];Li, Wen-yuan[4];Guo, Juan[1];Cui, Guang-hong[1];Huang, Lu-qi[1]

第一作者：Chen, Ling-li

通信作者：Cui, GH[1];Huang, LQ[1]

机构：[1]China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Dao di Herbs, Beijing 100700, Peoples R China;[2]Guangdong Pharmaceut Univ, Sch Tradit Chinese Med, Guangzhou 510006, Peoples R China;[3]Gansu Univ Tradit Chinese Med, Lanzhou 730000, Gansu, Peoples R China;[4]Qinghai Univ, Med Coll, Tibetan Med Res Ctr, Xining 810001, Peoples R China

第一机构：China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Dao di Herbs, Beijing 100700, Peoples R China

通信机构：[1]corresponding author), China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Dao di Herbs, Beijing 100700, Peoples R China.

年份：2021

卷号：193

外文期刊名：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000600773600019)】；

基金：This work was financially supported by the National Key R&D Program of China (2018YFA0900600), National Natural Science Foundation of China (Nos. 81891010, 81891013 and 81822046) and Key project at central government level: The ability to establish sustainable use of valuable Chinese medicine resources (2060302).

语种：英文

外文关键词：Aconitumplants; Different plant parts; UHPLC-Q-TOF-MS; Chemical constituents

摘要：Aconitum L., the main source of Aconitum medicinal materials, is rich in diterpenoid alkaloids. Several drugs derived from diterpenoid alkaloids are widely used to the current clinical treatment of pain, inflammation, and other symptoms. This paper aims to clarify the main metabolites and distribution of diterpenoid alkaloids in different parts of Aconitum plants. To that end, 7 species of Aconitum from three subgenera were analyzed by UHPLC-Q-TOF-MS under identical conditions. The fragmentation regularity of various types of diterpene alkaloids were determined and a total of 126 metabolites were identified by comparing the reference material and secondary mass spectrometry, with the literature. 67, 49, 17, 41, 14, 17 and 21 metabolites were identified from Aconitum carmichaeli, Aconitum stylosum, Aconitum sinomontanum, Aconitum vilmorinianum, Aconitum pendulum, Aconitum tanguticum and Aconitum gymnandrum, respectively. Meanwhile, the structure type of A. carmichaeli, A. stylosum, A. vilmorinianum, A. pendulum, A. gymnandrum were identified as C-19 type, A. sinomontanum was C-18 type, while A. tanguticum was C-20 type. A high similarity of metabolites was found between A. stylosum and A. vilmorinianum. The quantitative analysis of 19 compounds and the relative peak area of all metabolites which obtained through internal standard berberine, highlighted compounds like karakoline, talatisamine and atisine as references for future study of metabolic pathways. Furthermore, results from metabolites distribution and relative peak area analysis suggest that the leaf of A. carmichaeli, the leaf and stem of A. stylosum and A. vilmorinianum, and the flower of A. pendulum have potential as medicinal resources and are worth further development. These results establish a foundation for the comprehensive utilization of Aconitum resources. (C) 2020 Elsevier B.V. All rights reserved.