文献类型：期刊文献

英文题名：Establishment and characterization of the PDAC-X3 cell line: a novel Chinese-origin pancreatic ductal adenocarcinoma cell line

作者：Chai, Changpeng[1,2];Tang, Huan[3];Miao, Xin[4];Su, Yuanhui[3];Li, Lu[1];Yu, Cheng[3,5];Yi, Jianfeng[6];Ye, Zhenzhen[6];Miao, Long[1,3];Zhang, Bo[1];Wang, Zhengfeng[1,2];Luo, Wei[1];Hu, Jinjing[1];Zhang, Hui[3,7];Zhou, Wence[3,7];Xu, Hao[1,2,4]

第一作者：Chai, Changpeng

通信作者：Xu, H[1];Xu, H[2];Zhang, H[3];Zhou, WC[3];Xu, H[4];Zhang, H[5];Zhou, WC[5]

机构：[1]Lanzhou Univ, Hosp 1, Dept Gen Surg 4, 1 Donggang West Rd, Lanzhou 730000, Peoples R China;[2]Lanzhou Univ, Clin Med Sch 1, Lanzhou 730000, Peoples R China;[3]Lanzhou Univ, Clin Med Sch 2, Lanzhou 730000, Peoples R China;[4]Zhejiang Chinese Med Univ, Zhejiang Prov Hosp Chinese Med, Sch Clin Med 1, Hangzhou 310006, Peoples R China;[5]Lanzhou Univ, Dept Anesthesiol, Hosp 2, Lanzhou 730000, Peoples R China;[6]Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou 730000, Peoples R China;[7]Lanzhou Univ, Dept Gen Surg, Hosp 2, Lanzhou 730000, Peoples R China

第一机构：Lanzhou Univ, Hosp 1, Dept Gen Surg 4, 1 Donggang West Rd, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Hosp 1, Dept Gen Surg 4, 1 Donggang West Rd, Lanzhou 730000, Peoples R China;[2]corresponding author), Lanzhou Univ, Clin Med Sch 1, Lanzhou 730000, Peoples R China;[3]corresponding author), Lanzhou Univ, Clin Med Sch 2, Lanzhou 730000, Peoples R China;[4]corresponding author), Zhejiang Chinese Med Univ, Zhejiang Prov Hosp Chinese Med, Sch Clin Med 1, Hangzhou 310006, Peoples R China;[5]corresponding author), Lanzhou Univ, Dept Gen Surg, Hosp 2, Lanzhou 730000, Peoples R China.

年份：2024

外文期刊名：HUMAN CELL

收录：;Scopus(收录号：2-s2.0-85198729227);WOS:【SCI-EXPANDED(收录号:WOS:001268750600001)】；

基金：We extend our gratitude to Bullet Edits (http://www.bulletedits.cn) for their English language editing of the manuscript.DAS:The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.

语种：英文

外文关键词：Pancreatic cancer; Cell line establishment; Xenografted tumor; Short tandem repeat analysis; Drug sensitivity

摘要：In this study, a novel pancreatic cancer cell line, termed pancreatic ductal adenocarcinoma (PDAC)-X3 cell line, was successfully derived from the primary tumor. Comprehensive analyses of its malignant phenotype, molecular properties, specific biomarkers, and histological features confirmed that PDAC-X3 cells serve as a valuable model for investigating the underlying mechanisms driving pancreatic carcinogenesis and advancing potential therapeutic strategies. The newly established cell line was continuously cultured for over 12 months and was stably passaged through more than 50 generations. Morphologically, PDAC-X3 cells displayed characteristics typical of epithelial tumors. The population doubling time for PDAC-X3 cells was determined to be 50 h. Karyotype analysis revealed that 75% of PDAC-X3 cells presented as hypotriploid, while 25% were sub-tetraploid, with representative karyotypes being 53 and XY der (1) inv (9) der (22). In suspension culture, PDAC-X3 cells efficiently formed organoids. Upon inoculation into BALB/C nude mice, these cells initiated the development of xenograft tumors, achieving a tumor formation rate of 33%. Morphologically, these xenografted tumors closely resembled the primary tumor. Drug sensitivity assays indicated that PDAC-X3 cells exhibited resistance to oxaliplatin but demonstrated sensitivity to 5-Fluorouracil (5-FU), gemcitabine, and paclitaxel. Immunohistochemical analysis revealed that CK7, CK19, E-cadherin, Vimentin, CA19-9 were positively expressed in PDAC-X3 cells. Meanwhile, the expression rate for Ki-67 was 30%, and that for CEA was not detected. Our findings underscore that PDAC-X3 represents a novel pancreatic cancer cell line, positioning it as a valuable model for basic research and the advancement of therapeutic strategies against pancreatic cancer.