文献类型：期刊文献

英文题名：Extracts of Dracocephalum tanguticum Maxim Ameliorate Acute Alcoholic Liver Disease via Regulating Transcription Factors in Mice

作者：Guo, Min[1,2,3];Gu, Liwei[4];Hui, Heping[5];Li, Xiaodong[2,3];Jin, Ling[1]

第一作者：郭玫;Guo, Min

通信作者：Jin, L[1];Li, XD[2];Li, XD[3]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[2]Gansu Acad Tradit Chinese Med, Inst Chinese Mat Med, Lanzhou, Peoples R China;[3]Gansu Prov Hosp Tradit Chinese Med, Lab Chinese Med, Lanzhou, Peoples R China;[4]China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing, Peoples R China;[5]Gansu Agr Technol Coll, Lanzhou, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[2]corresponding author), Gansu Acad Tradit Chinese Med, Inst Chinese Mat Med, Lanzhou, Peoples R China;[3]corresponding author), Gansu Prov Hosp Tradit Chinese Med, Lab Chinese Med, Lanzhou, Peoples R China.|[10735]甘肃中医药大学;[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-85127823657);WOS:【SCI-EXPANDED(收录号:WOS:000777703000001)】；

基金：Funding This study was supported by the National Natural Science Foundation of China (82160818), the Natural Science Foundation of Gansu (20JR10RA349), the Gansu Scientific Research Project of Traditional Chinese Medicine (GZK-2018-55), and the Lanzhou Science and Technology Development Steering Programme (2019-ZD-122).

语种：英文

外文关键词：Dracocephalum tanguticum Maxim; acute alcoholic liver disease; transcription factors; Tibetan medicine; extract

摘要：Alcoholic liver disease (ALD) caused by excessive drinking is a health and economic concern worldwide. Given the high morbidity, mortality, and the progressive nature of ALD, finding effective interventions is essential. Previous studies have confirmed that edible food plants and their bioactive compounds exert a protective effect against ALD. Dracocephalum tanguticum Maxim (DTM) is one of the important traditional Tibetan medicines in China with the effect of clearing away liver heat, used for the treatment of hepatitis. In this study, the DTM chloroform extract (DtM-C), ethyl acetate extract (DtM-E), and n-butanol extract (DtM-B) were obtained by ethanol extraction combined with fractional extraction. Acute ALD was induced in mice given intragastric ethanol. Serum and liver biochemical markers were detected by ELISA. Liver histological observation, Oil Red O, and Masson's trichrome staining were performed. Liver injury cells were induced by ethanol. The cell vitality was detected by using MTT colorimetry. The expressions of Nrf2, NF-kappa B, STAT3, AP-1, CREB, HIF-1 alpha, HO-1, NQO-1, GSTA1, IKB2, and Keap1 were detected by real-time polymerase chain reaction (PCR) to elucidate the mechanism of hepatoprotective effect, and the results were verified by using Western blot. The results of serum liver function indicators (ALT, AST, and ADH), serum hepatic lipid indicators (TC, TG, HDL-C, and LDL-C), and lipid peroxidation indicators (ADH, MDA, SOD, CAT, and GSH-Px) in liver tissue and liver histological observation showed that DtM-E could improve liver function, alleviate fatty degeneration, edema, cell necrosis, and liver fibrosis caused by alcohol. DtM-E also increased the vitality of EtOH-induced liver injury cells, upregulated the mRNA expression of Nrf2, HO-1, NQO-1, and GSTA1, while downregulated the expression of Keap-1, p65, and NF-kappa B. Western blot results were consistent with PCR. The results suggest that DtM-E has a protective effect against ALD in vitro and in vivo, and its mechanism of action may be related to the activation of Nrf2/Keap-1 and inhibition of the P65/NF-kappa B signaling pathways.