文献类型：期刊文献

中文题名：多指标成分结合化学计量学综合评价甘肃产甘草的质量

英文题名：Comprehensive evaluation of quality of licorice(Glycyrrhiza uralensis) in different growing regions of Gansu Province based on multi-index components combined with chemometrics methods

作者：侯嘉[1];郭鸿儒[2];杨扶德[1];邵士俊[3]

第一作者：侯嘉

机构：[1]甘肃中医药大学药学院,甘肃省中药质量与标准研究重点实验室培育基地,甘肃兰州730000;[2]兰州工业研究院,甘肃兰州730070;[3]中国科学院兰州化学物理研究所中国科学院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）|甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2019

卷号：50

期号：16

起止页码：3923

中文期刊名：中草药

外文期刊名：Chinese Traditional and Herbal Drugs

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家中医药行业科研专项课题:国家基本药物所需中药材种子种苗繁育基地建设(财社〔2012〕13号);甘肃省普通中医药科研项目(GZK-2011-63);甘肃省科技厅“三区”人才科技支持项目(2016-13);甘肃省高等学校科研项目(2015B-072)

语种：中文

中文关键词：甘草;多指标成分;因子分析;聚类分析;质量评价

外文关键词：licorice;multi-index components;factor analysis;cluster analysis;quality evaluation

摘要：目的选取甘肃代表性区域(河西、陇中、陇东地区)野生和栽培甘草进行多指标成分测定并结合化学计量学综合分析甘肃产甘草质量,为评价适宜生产区域提供依据。方法建立了多波长检测HPLC测定甘草样品中7种三萜类和黄酮类成分(甘草酸、甘草苷、异甘草苷、芹糖甘草苷、芹糖异甘草苷、甘草素、异甘草素)含量的方法,结合因子和聚类分析综合评价甘肃代表性区域产甘草样品的质量。结果因子分析中,第1公因子与甘草酸、甘草苷、异甘草苷有较强的相关性,第2公因子与甘草素、异甘草素有较强的相关性,2个公因子的方差累积贡献率达到84.28%,能较全面地反映甘草的质量;聚类分析结果将25批不同区域甘草质量分为3类:I类甘草质量最好,主要是野生甘草及产自内蒙古杭锦旗的甘草;Ⅱ类甘草质量较好,主要产自甘肃河西地区及甘肃陇西;Ⅲ类甘草质量较差,主要产自甘肃陇中地区(白银)。结论甘肃河西地区及陇西较适宜大力发展甘草产业。通过多指标成分结合化学计量法可用于进一步评价甘草质量,判别适宜甘草生产的产地区划。
Objective In this study,the wild and cultivated licorice of Gansu representative regions(Hexi,Longzhong and Longdong)were measured and analyzed by multi-index components combined with chemometrics methods,which will provide scientific basis for the evaluation of the quality of licorice and the suitable producing areas in Gansu Province.Methods Seven main bioactive components(glycyrrhizin,liquiritin,isoliquiritin,liquiritin apioside,isoliquiritin apioside,liquiritigenin and isoliquiritigenin)in 25 batches of licorice samples were simultaneously determined by HPLC-DAD to comprehensively evaluate the quality of licorice combined with factor analysis and cluster analysis.Results Factor analysis showed that there was a strong correlation between the first common factor and glycyrrhizin,liquiritin and isoliquiritin;and between the second common factor and liquiritigenin,isoliquiritigenin.The variance contribution rate of two common factors was 84.28%,which reflected licorice quality overall.And cluster analysis showed that the quality of 3-year-old licorice(Glycyrrhizae Radix)cultivated in Hangjinqi of Inner Mongolia was the best,followed by that cultivated in Hexi and Longxi of Gansu Province.The quality of licorice in Longzhong of Gansu Province was worse.Conclusion These results showed that it was more suitable to develop licorice planting in Longxi and Hexi area in Gansu Province.The overall evaluation of multi-index components and chemometrics has reference value for quality control of licorice and optimization its suitable planting areas.