文献类型：期刊文献

中文题名：参苓白术散对脾虚湿困型溃疡性结肠炎大鼠结肠p38MAPK及TNF-α、IL-4的干预作用

英文题名：Effect of Shenling Baizhu Powder on the Colonic Expression of p38MAPK,TNF-αand IL-4 in UC Rats with the Pattern of Spleen Deficiency and Dampness Retention

作者：贾育新[1];毕殿勇[2];段永强[1];明海霞[1];万生芳[1];程小丽[1];成映霞[1];呼会茹[1]

第一作者：贾育新

机构：[1]甘肃中医药大学,甘肃兰州730020;[2]上海中医药大学,上海100006

第一机构：甘肃中医药大学

年份：2018

卷号：46

期号：5

起止页码：11

中文期刊名：中医药学报

外文期刊名：Acta Chinese Medicine and Pharmacology

收录：CSTPCD

基金：国家自然科学基金项目(81360518)

语种：中文

中文关键词：溃疡性结肠炎;参苓白术散;TNF-α;p38MAPK;IL-4;基因;蛋白

外文关键词：Ulcerative colitis ; Shenling Baizhu Powder; TNF - α; p38mMAPK ; IL - 4 ; Gene ; Protein

摘要：目的:研究参苓白术散对脾虚湿困型溃疡性结肠炎大鼠结肠组织p38MAPK、TNF-α及IL-4基因、蛋白表达的干预作用。方法:SPF级Wistar大鼠60只,按随机数字表法标记,按性别不同分笼饲养。取雌、雄大鼠各5只作为空白组,其余大鼠依据性别不同分笼饲养;采用复合因素+TNBS/乙醇刺激法(乙醇浓度50%)构建脾虚湿困型UC大鼠模型;造模成功后,依随机数字表法将模型大鼠分为模型组、参苓白术散低、中、高治疗组,剂量分别为6 g/(kg·d)、12 g/(kg·d)、24 g/(kg·d)及阳性对照组,柳氮磺胺吡啶组,SASP组0. 2 g/(kg·d),每组各10只,雌雄各半、分笼饲养。其余各组大鼠均以蒸馏水灌胃治疗,剂量:10 g/(kg·d),疗程3周,每日灌胃1次。免疫组化法检测各组实验大鼠结肠组织p38MAPK、TNF-α、IL-4的蛋白含量表达; RT-PCR法检测UC各组大鼠结肠组织内p38MAPK、TNF-α及IL-4的基因表达水平。结果:与空白组比较,模型组大鼠结肠组织内p38MAPK、TNF-α蛋白含量、基因表达水平明显升高(P <0. 01或P <0. 05); IL-4的含量蛋白、基因表达水平显著降低(P <0. 01或P <0. 05);与模型组相比较,阳性对照组、参苓白术散中、高剂量组大鼠p38MAPK、TNF-α基因、蛋白表达水平降低显著(P <0. 01或P <0. 05); IL-4的蛋白含量、基因表达水平明显升高(P <0. 01或P <0. 05),尤以参苓白术散高剂量组及阳性对照组作用显著。结论:参苓白术散可明显调节脾虚湿困型UC大鼠病变部位结肠组织内p38MAPK、TNF-α及IL-4基因及蛋白表达的水平。
Objective： To study the effect of Shenling Baizhu Powder on colonic protein and mRNA expressions of p38MAPK, TNF -α and IL -4 in rats with ulcerative colitis（UC） of the pattern of Spleen deficiency and dampness re- tention. Methods： 60 SPF Wistar rats were assigned randomly and fed in different groups according to gender. Five fe- male rats and five male rats were assigned to the blank group; others were fed in different cages according to gender. The UC model was established by combination method （TNBS/50% ethanol）. The successful models were randomly di- vided into the model group, Shenling Baizhu Powder groups（6 g/kg · d, 12 g/kg · d, 24 g/kg · d）, and SASP group （sulfasalazine, 0.2 g/kg · d） ; with 10 rats in each group. Rats in the blank and the model group were intragastrically with distilled water （10 g/kg ·d ）. The treatment course was thine weeks. The protein and mRNA expessions of p38MAPK, TNF-α and IL-4 in colonic tissues were detected by inmmnohistochemistry and RT- PCR respectively. Results： Compared to the control group, the protein and mRNA expressions of p38MAPK and TNF- α were significantly increased in the model group（P 〈 0.01, P 〈 0.05） ; the protein and mRNA expressions of IL- 4 were significantly de- creased （P 〈 0.01, P 〈 0.05）. Compaared to the model group, protein and mRNA expressions of p38MAPK and TNF - αwere significantly decreased in SASP group and in Shenling Baizhu Powder roups of medium - dose and high - dose （P 〈 0.01, P 〈 0.05） ; the protein and mRNA expressions of IL -4 were significantly increased in the SASP group and Shengling Baizhu Powder group of high - dose（P 〈 0.01, P 〈 0.05 ）. Conclusion ： Shenling Baizhu Powder can signifi- cantly regulate the protein and mRNA expressions of p38MAPK, TNF -αand IL - 4 in UC rats with the pattern of Spleen deficiency and dampness retention.