文献类型：期刊文献

中文题名：甘肃省黄芩灰霉病的发生及其病原鉴定

英文题名：Pathogen Identification of Gray Mold on Scutellaria baicalensis in Gansu Province

作者：王艳[1];杜弢[1];曾翠云[1];陈红刚[1]

第一作者：王艳

机构：[1]甘肃中医药大学药学院,甘肃兰州730030

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2017

卷号：19

期号：12

起止页码：1735

中文期刊名：中国现代中药

外文期刊名：Modern Chinese Medicine

收录：CSTPCD

基金：国家自然基金项目(31460013);甘肃省自然基金(17JR5RA164);甘肃省科技计划基础研究创新群体(1606RJIA323);中央财政引导地方科技创新平台项目;省自然基金(1508RJZA011)

语种：中文

中文关键词：黄芩;灰霉病;形态学;系统发育分析;病原鉴定

外文关键词：Scutellaria baicalensis ; gray mold; morphology; phylogenetic analysis ; pathogen identification

摘要：目的:据2013—2016年调查,甘肃省黄芩灰霉病发生严重,常年发病率为10~15%,严重度2~3级。本研究旨在明确该病在甘肃省的发生情况及其病原菌的分类地位,为该病的防控提供一定的理论依据。方法:通过植物病害田间调查确定病害发生情况,将形态学和分子生物学方法相结合确定病原分类地位。结果:黄芩灰霉病症状主要表现为在叶片和茎秆上形成褐色病斑,严重时整株叶片干枯,植株死亡。病原菌分生孢子梗褐色,多隔,长宽450.0~716.6μm×9.4~18.8μm;分生孢子长椭圆形至椭圆形,无色,大小8.2~16.5μm×5.9~8.2(平均10.6×7.3)μm。基于形态学和r DNA ITS基因片段构建的系统发育树分析,将黄芩灰霉病病原鉴定为灰葡萄孢(Botrytis cinerea Pers.)。结论:黄芩灰霉病在甘肃省各个黄芩产区均有发生,依据病害传播规律,将栽培措施和药剂喷施相结合进行病害的防治。
Objective：During disease surveys on medicinal plants in Gansu province in 2013-2016,we found that gray mold on Scutellaria baicalensis was a serious disease with 10~15% disease incidence and 2~3 level of disease severity.The study of the disease incidence and the pathogen identification will provide scientific basis to the control of the disease.Methods：The disease incidence in Gansu province was determined by field surveys.Morphological and molecular methods were combined to identify the pathogen.Results：The disease leads to leaf lesions and plant blight.The conidiophore of the causal pathogen is brown,multi septate,450.0~716.6 μm×9.4~18.8 μm in size;the conidia is aseptate,colorless,ellipsoidal,8.2~16.5 μm ×5.9~8.2（mean=10.6×7.3）μm in size.Based on morphological characteristics and rDNA internal transcribed spacer sequence analysis the fungi is identified as Botrytis cinerea Pers.S.baicalensis gray mold appears in all the planting areas in Gansu province.Conclusion：The cultivation methods and chemical control methods should be combined to control this disease.