文献类型：期刊文献

英文题名：Exploring the Targets of Gulao Yukang Pill in Rheumatoid Arthritis via the Hippo Signaling Pathway: An Integrated Network Pharmacology and Experimental Validation Study

作者：Wang, Tao[1];Wang, Gang[1];Wang, Jia[1];Liu, Hailong[2];Jiang, Ganggang[1,1,2,2]

第一作者：王涛

通信作者：Wang, G[1]

机构：[1]Gansu Univ Chinese Med, Affiliated Hosp, Lanzhou, Peoples R China;[2]Gansu Univ Chinese Med, Lanzhou, Peoples R China

第一机构：甘肃中医药大学第二附属医院

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Affiliated Hosp, Lanzhou, Peoples R China.|[10735b845793de6ae2b30]甘肃中医药大学第二附属医院;[10735]甘肃中医药大学;

年份：2025

卷号：28

期号：8

外文期刊名：INTERNATIONAL JOURNAL OF RHEUMATIC DISEASES

收录：;Scopus(收录号：2-s2.0-105011975692);WOS:【SCI-EXPANDED(收录号:WOS:001537590200001)】；

基金：This research was supported by the Gansu Provincial Science and Technology Program (23JRRA1584, 24JRRA548) and the Gansu Provincial Traditional Chinese Medicine Research Project (GZKP-2022-18). AI-assisted tools were solely used for grammar checking during manuscript preparation. All scientific content was generated by the authors.

语种：英文

外文关键词：Gulao Yukang pill; Hippo signaling pathway; network pharmacology; rheumatoid arthritis; treatment

摘要：ObjectiveInvestigating the Mechanism of Gulao Yukang Pill (GLYK) in Treating Rheumatoid Arthritis (RA) via the Hippo signaling pathway and Its Regulatory Effects on Th17/Treg Cell Balance.MethodsNetwork pharmacology was applied to construct a network mapping the interactions between drug active ingredients and target genes, pinpointing the crucial active components and genetic targets through which GLYK exerts its effects on RA. Using Gene Set Variation Analysis (GSVA) in R4.2.2, we predicted the pathway activity scores of 7 Hippo pathways between the disease and control groups and selected those with significant differences. Key genes were correlated with the selected pathways, and target genes related to the Hippo pathway were screened. The binding capacity of crucial active components and their corresponding target genes was predicted using the Deep Purpose algorithm framework. Targets acting on RA were screened, and a CIA (Collagen-Induced Arthritis) animal model was developed for the purpose of further demonstrating the therapeutic impact of GLYK on RA and to verify the results of network pharmacology through histopathological observation, ELISA method detection, flow cytometry detection, Western blot detection, and qRT-PCR detection.ResultsNetwork pharmacology has identified nine key targets in the Hippo pathway associated with rheumatoid arthritis (RA) treatment, including Mst1 and TAZ, which are critical for GLYK's therapeutic effects as they are linked to its core effective ingredients that mediate the treatment of RA. Animal experiments validated the network pharmacology predictions, confirming that Mst1 and TAZ in the Hippo pathway are pivotal therapeutic targets for RA. GLYK suppresses inflammation and bone destruction by inhibiting the Hippo pathway components Mst1/TAZ, thereby reducing the Th17/Treg ratio.ConclusionGLYK can influence the expression of upstream core molecules Mst1 and downstream effector molecules TAZ in the Hippo pathway, which can reduce the swelling and arthritis index of CIA rats, lower the proportion of Th17/Treg cells, inhibit inflammation, and bone destruction. The Hippo pathway is a goal of GLYK in the intervention of RA.