文献类型：期刊文献

英文题名：Development and validation of a simple ultra-high-performance-tandem mass spectrometry method for the simultaneous determination of five bioactive components in rat plasma of Hedysari Radix

作者：Luo, Xudong[1];Li, Chengyi[1,2];Qi, Peng[1];Liang, Tingting[1];Feng, Xiaoli[1];Wang, Mingwei[1];Liu, Shubin[1];Qiang, Zhengze[1];Jia, Miaoting[1];Wei, Xiaocheng[1];Li, Xu[1];He, Jungang[1];Wang, Yan[1]

第一作者：Luo, Xudong

通信作者：Li, CY[1]

机构：[1]Gansu Univ Chinese Med, Dept Pharm, Lab Identificat & Qual Evaluat Chinese Med Herbs, Lanzhou, Peoples R China;[2]Gansu Univ Chinese Med, Dept Pharm, Lab Identificat & Qual Evaluat Chinese Med Herbs, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Dept Pharm, Lab Identificat & Qual Evaluat Chinese Med Herbs, Lanzhou 730000, Gansu, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2024

外文期刊名：SEPARATION SCIENCE PLUS

收录：EI(收录号：20241015678233);Scopus(收录号：2-s2.0-85186473268);WOS:【ESCI(收录号:WOS:001169615700001)】；

基金：This work was financially supported by the National Nature Science Foundation of China (No. 82160730); the Key R & D Plan Project of Gansu Province (21YF5FA133); and the "Double First-Class" Scientific Research Key Project (GSSYLXM-05).

语种：英文

外文关键词：formononetin; Hedysari Radix; medicarpin; ononin; UHPLC-MS/MS

摘要：Hedysari Radix is a commonly used traditional Chinese medicine that improves immunity; formononetin, ononin, calycosin, medicarpin, and vanillic acid play an important role in achieving this effect. Herein, a precise and sensitive ultra-high-performance-tandem mass spectrometry method was developed and validated for the determination of five bioactive components that were extracted from plasma using a protein precipitation method. A Waters CORTES C18 (4.6 x 50 mm, 2.7 mu m) and a mobile phase composed of methanol and water (containing 0.2% formic acid) were used for the separation. The method was linear within the concentration range of 19.53-625.00 ng/mL for formononetin; 0.01-3.13 ng/mL for ononin; 0.01-3.13 ng/mL for calycosin; 0.16-5.00 ng/mL for medicarpin; and 19.53-625.00 ng/mL for vanillic acid. This method has a lower limit of quantification, is simple, and has a short analysis time and a high degree of separation. The intra-day precisions and inter-day precisions of quality control samples were traced to be below 8.58% and 12.64%, respectively. Fidelity intervened from -8.61% to 10%. Finally, the developed method was used to determine the concentrations of the five bioactive components in rat plasma after the administration of rubbing- and non-rubbing processed Hedysari Radix.