文献类型：期刊文献

中文题名：和厚朴酚对非小细胞肺癌细胞的辐射增敏效应

英文题名：Radiosensitizing Effect of Honokiol on Non-small Cell Lung Carcinoma Cells

作者：蔺兴遥[1,2];刘炳涛[3,4];马晓辉[1,2];张扬[1,2];陈卫强[3,4];金晓东[3,4]

第一作者：蔺兴遥

机构：[1]甘肃中医药大学科研实验中心,兰州730000;[2]敦煌医学与转化教育部重点实验室,兰州730000;[3]中国科学院近代物理研究所,兰州730000;[4]中国科学院大学核科学与技术学院,北京100049

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2019

卷号：36

期号：1

起止页码：104

中文期刊名：原子核物理评论

外文期刊名：Nuclear Physics Review

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学基金资助项目(U1730133);甘肃省自然科学基金资助项目(17JRSRA310)~~

语种：中文

中文关键词：和厚朴酚;非小细胞肺癌细胞;X射线;碳离子;辐射增敏

外文关键词：Honokiol;non-small cell lung carcinoma cells;X-rays;carbon ions;radiosensitization

摘要：研究了和厚朴酚(HNK)对非小细胞肺癌(NSCLC)细胞系A549和H1299对低线性能量转移(LET)X射线和高LET碳离子的辐射增敏效应。首先用CCK-8检测了HNK对A549和H1299细胞的生长抑制情况,发现20μmol/L的HNK处理对细胞的生长抑制作用较弱。用该浓度HNK预处理细胞2 h后给予不同剂量X射线或碳离子的照射,克隆存活法检测细胞的辐射敏感性,Annexin-PI双染法检测细胞凋亡,γH2AX焦点法检测DNA的双链断裂(DSB)损伤。实验结果显示:与X射线相比,NSCLC细胞对碳离子更敏感,HNK预处理仅对碳离子照射有辐射增敏作用;与碳离子单独照射相比,HNK预处理联合碳离子照射诱导了更明显的细胞凋亡;在照射后24 h,HNK预处理联合碳离子照射引起的细胞γH2AX焦点阳性率维持在较高水平,而X射线照射没有这些效应。实验结果表明,HNK预处理抑制了NSCLC细胞DNA的DSB修复,诱导了细胞凋亡的发生,从而提高了细胞对碳离子的辐射敏感性。
The radiosensitizing effect of Honokiol(HNK) on non-small cell lung carcinoma(NSCLC) cell lines A549 and H1299 to low-linear energy transfer(LET) X-rays and high-LET carbon ions was investigated in this study. First, the inhibitory effects of HNK on the growth of A549 and H1299 cells were detected by CCK-8 assay, and 20 μmol/L HNK treatment was found to induce a growth inhibitory effect slightly in these two cell lines. Cells were pre-treated with HNK and then irradiated with X-rays and carbon ions of different doses.Cellular radiosensitivity, apoptosis and DNA damage were analyzed by clonogenic survival, Annexin-PI staining and γH2 AX foci, respectively. The results showed the cells were more sensitive to carbon ion irradiation compared to X-rays and the radiosensitization of HNK was only observed after carbon ion irradiation. Furthermore, the co-treatment led to higher apoptosis rate 48 h after irradiation and increased the positive rate of γH2 AX foci 24 h after irradiation in A549 and H1299 cells compared with those in the groups treated with carbon ion irradiation alone. These phenomena were not observed after X-ray irradiation. Our data suggest that the pre-treatment with HNK inhibited DNA DSB repair, induced apoptosis and then enhanced the cellular radiosensitivity to carbon ions in NSCLC cells.