文献类型：期刊文献

中文题名：阿尔茨海默病大鼠认知功能损害和肠道菌群改变的关联性分析及针刺“益智调神”穴方的调节作用

英文题名：Analysis of the correlation between cognitive impairment and intestinal flora change in Alzheimer’s disease rats and the regulatory effect of acupuncture at the acupoints for‘Yizhi Tiaoshen’

作者：李丽[1];张鑫[2];马翠[1];魏玉婷[1];严兴科[1,3]

第一作者：李丽

机构：[1]甘肃中医药大学针灸推拿学院,兰州730000;[2]兰州大学第一医院康复科,兰州730000;[3]甘肃省中医药研究中心,兰州730000

第一机构：甘肃中医药大学针灸推拿学院

年份：2025

卷号：40

期号：5

起止页码：2225

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：;北大核心:【北大核心2023】；

基金：甘肃中医药大学引进人才科研启动基金项目(No.2024YJRC-06);甘肃省中医药研究中心2024年度专项开放课题(No.zyzx-2024-zx03);2023年甘肃省省级重点人才项目(No.甘组通字[2023]20号)。

语种：中文

中文关键词：阿尔茨海默病;“益智调神”穴方;针刺;认知功能;肠道菌群;机制

外文关键词：Alzheimer’s disease(AD);Acupoints for‘Yizhi Tiaoshen’;Acupuncture;Cognitive function;Intestinal flora;Mechanism

摘要：目的:检测分析阿尔茨海默病(AD)大鼠认知功能损害和肠道菌群改变的关联性及针刺的调节作用,探讨“益智调神”穴方针刺干预AD的效应机制。方法:运用腹腔注射D-半乳糖联合海马CA1区注射冈田酸复制AD模型,随机分为空白组、假手术组、模型组、西药组和针刺组。西药组给予盐酸多奈哌齐溶液灌胃治疗,针刺组选取“益智调神”穴方针刺治疗。Morris水迷宫评估认知功能,16S rRNA技术检测肠道菌群变化,Pearson相关性分析丰度显著差异菌属与认知功能的相关性。结果:与假手术组比较,模型组逃避潜伏期延长(P<0.05),在原平台象限停留时间和穿越原平台次数减少(P<0.05);肠道菌群α多样性增加(P<0.05);β多样性模型组菌群分布较分散,远离空白组和假手术组;门水平拟杆菌门丰度增多,厚壁菌门丰度下降(P<0.05);属水平普氏菌属、普雷沃菌科_NK3B31群、普雷沃氏菌科_UCG-001丰度增加,梭状芽胞杆菌属UCG-014、穆里氏菌科丰度下降(P<0.05)。与模型组比较,西药组和针刺组逃避潜伏期缩短(P<0.05),在原平台象限停留时间和穿越原平台次数增加(P<0.05);肠道菌群α多样性下降(P<0.05);β多样性西药组和针刺组肠道菌群重叠,与空白组和假手术组接近;门水平拟杆菌门丰度下降、厚壁菌门丰度增多(P<0.05);属水平普氏菌属、普雷沃菌科_NK3B31、普雷沃氏菌科_UCG-001丰度下降,瘤胃球菌属丰度增加(P<0.05)。针刺组与西药组比较,各项指标差异无统计学意义。Pearson相关性分析表明,拟杆菌门、普氏菌属、普雷沃氏菌科_UCG-001和普雷沃氏菌科_NK3B31群丰度与认知功能呈负相关(P<0.05),厚壁菌门丰度与认知功能呈正相关(P<0.05)。结论:AD大鼠肠道菌群结构明显失衡,多样性和丰度异常改变,且与认知功能损害存在一定关联性,“益智调神”穴方针刺可显著改善AD大鼠认知功能,其作用机制可能与调节和恢复肠道微生态平衡有关。
Objective:To detect and analyze the correlation between cognitive impairment and intestinal flora change in Alzheimer’s disease(AD)rats and the regulatory effect of acupuncture,and to explore the effect mechanism of acupuncture at the acupoints for‘Yizhi Tiaoshen’in AD.Methods:The AD model was replicated by intrabitoneal injection of D-galactose combined with hippocampal CA1 injection of Okadaic acid.The model was then randomly divided into the following groups:control group,sham operation group,model group,medication group,and acupuncture group.The medication group received gavage treatment with Donepezil hydrochloride solution,while the acupuncture group underwent treatment of the acupuncture at the acupoints for‘Yizhi Tiaoshen’.Cognitive function was assessed using Morris water maze,changes in intestinal flora were detected using 16S rRNA technology,and Pearson correlation analysis was conducted to examine the relationship between bacterial genera with significant differences in abundance and cognitive function.Results:Compared with the sham operation group,the escape latency of the model group was prolonged(P<0.05),the residence time in the original platform quadrant and the number of crossing the original platform were reduced(P<0.05);αdiversity of intestinal flora increased(P<0.05);the distribution ofβdiversity of intestinal flora in model group was more dispersed than that in control group and sham operation group;the abundances of Bacteroidota increased and Firmicutes decreased(P<0.05);the abundances of Prevotella,Prevotellaceae_NK3B31_group and Prevotellaceae_UCG-001 increased,while the abundances of Clostridia_UCG-014 and Muribaculaceae decreased(P<0.05).Compared with model group,the escape latency of medication group and acupuncture group was shortened(P<0.05),and the residence time in the original platform quadrant and the number of crossing the original platform were increased(P<0.05);αdiversity of intestinal flora decreased(P<0.05);βdiversity of intestinal flora in medication group and acupuncture group overlapped,which was close to that of control group and sham operation group;the abundances of Bacteroidota decreased and Firmicutes increased(P<0.05);the abundances of Prevotella,Prevotellaceae_NK3B31_group and Prevotellaceae_UCG-001 decreased,while the abundances of Ruminococcus increased(P<0.05).There was no statistical significance between acupuncture group and medication group.Pearson correlation analysis showed that the abundances of Bacteroidota,Prevotella,Prevotellaceae_UCG-001 and Prevotellaceae_NK3B31_group were negatively correlated with cognitive function(P<0.05),while the abundances of Firmicutes were positively correlated with cognitive function(P<0.05).Conclusion:The structure of intestinal flora in AD rats is obviously unbalanced,with abnormal changes in diversity and abundance,and there is a certain correlation with cognitive impairment.Acupuncture at the acupoints for‘Yizhi Tiaoshen’can significantly improve the cognitive function of AD rats,and its mechanism may be related to regulating and restoring the intestinal microecological balance.