文献类型：期刊文献

中文题名：老年类风湿关节炎患者血清25-羟维生素D_(3)水平与肠道微生态的关系探讨

英文题名：Relationship between serum 25 hydroxyvitamin D_(3) and intestinal microecology in elderly patients with rheumatoid arthritis

作者：刘海龙[1];王钢[1];王佳[1];王涛[1];田杰祥[1];王丽琴[1];杨芳[1];魏勇[1];郭鸿玲[1]

第一作者：刘海龙

机构：[1]甘肃中医药大学附属医院风湿骨病科,甘肃兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2021

卷号：33

期号：9

起止页码：1045

中文期刊名：中国微生态学杂志

外文期刊名：Chinese Journal of Microecology

收录：CSTPCD;;CSCD:【CSCD_E2021_2022】；

基金：甘肃省中医药管理局科技计划项目(G2K-2019.34);国家自然科学基金地区项目(81960832)(临床预实验)。

语种：中文

中文关键词：老年;类风湿关节炎;血清25-羟维生素D_(3)肠道微生态

外文关键词：Elderly;Rheumatoid arthritis;Serum 25 hydroxyvitamin D_(3);Intestinal microecology

摘要：目的探讨老年类风湿关节炎(RA)患者血清25-羟维生素D_(3)[25(OH)D_(3)]水平与肠道微生态的关系。方法回顾性分析2018年8月至2020年4月甘肃中医药大学附属医院风湿骨病科住院收治的83例老年RA患者的临床资料,并选择同期进行体检的80例健康者的体检资料,分别记为A组、B组。对比2组研究对象血清25(OH)D_(3)水平以及肠道微生物菌群组成;采用Pearson相关性分析,分析老年RA患者血清25(OH)D_(3)水平与肠道微生物菌群丰富度的相关性。结果 A组患者血清25(OH)D_(3)水平明显低于B组(t=23.297,P<0.05);A组患者肠道微生物菌群Chaol指数、Observed species指数和Shannon指数均低于B组(t=11.258、14.681、22.157,均P<0.05);A组患者肠道微生物菌群拟杆菌门、放线菌门、变形菌门、普雷沃菌属、梭状芽胞杆菌属、戈登菌属的相对丰度均高于B组(t=19.472、12.011、27.354、20.304、15.637、24.414,均P<0.05),厚壁菌门、乳杆菌属、类杆菌属、嗜血杆菌属、韦荣球菌属、真杆菌属的相对丰度均低于B组(t=10.095、3.663、6.787、12.848、7.786、9.122,均P<0.05);RA患者25(OH)D_(3)水平与厚壁菌门、乳杆菌属、类杆菌属、嗜血杆菌属、韦荣球菌属、真杆菌属的相对丰度呈正相关(r=8.126、8.031、8.415、8.057、8.236、8.467,均P<0.05),与拟杆菌门、放线菌门、变形菌门、普雷沃菌属、梭状芽胞杆菌属、戈登菌属呈负相关(r=-7.639、-7.856、-8.135、-8.127、-7.536、-7.865,均P<0.05)。结论 RA患者血清25(OH)D_(3)水平降低,肠道微生物菌群拟杆菌门、放线菌门、变形菌门、普雷沃菌属、梭状芽胞杆菌属、戈登菌属的相对丰度增加,厚壁菌门、乳杆菌属、类杆菌属、嗜血杆菌属、韦荣球菌属、真杆菌属的相对丰度减少,老年RA患者血清25(OH)D_(3)水平与肠道微生物菌群分布相关。
Objective To explore the relationship between the level of serum 25 hydroxyvitamin D [25(OH)D_(3)] and intestinal microecology in elderly patients with rheumatoid arthritis(RA). Methods The clinical data of 83 RA patients in our department from August 2018 to April 2020(group A) were retrospectively analyzed, and the physical examination data of 80 healthy volunteers who underwent physical examination at the same time were recorded as group B. Serum 25(OH)D_(3)levels were compared between the two groups. Pearson correlation analysis was used to analyze the correlation between serum 25(OH)D_(3)level and intestinal floral richness in elderly RA patients. Results The serum 25(OH)D_(3)level in group A was significantly lower than that in group B(t=23.297, P<0.05). The indexes of Chaol, Observed species and Shannon in group A were lower than those in group B(t=11.258, 14.681, 22.157, P<0.05) respectively. The relative richness of Bacteroidetes, Actinomycetes, Proteobacteria, Prevotella, Clostridium and Gordonia in group A were higher(t=19.472, 12.011, 27.354, 20.304, 15.637, 24.414, P<0.05), while those of Firmicutes, Lactobacillus, Bacteroides, Haemophilus, Veillonella and Eubacterium were lower than those in group B(t=10.095, 3.663, 6.787, 12.848, 7.786, 9.122, P<0.05), respectively. The level of 25(OH)D_(3)in RA patients was positively correlated with the relative richness of Firmicutes, Lactobacillus, Bacteroides, Haemophilus, Veillonella and Eubacterium(r=8.126, 8.031, 8.415, 8.057, 8.236, 8.467, P<0.05), and negatively correlated with Bacteroides, Actinomycetes, Proteobacteria, Prevotella, Clostridium and Gordonia(r=-7.639,-7.856,-8.135,-8.127,-7.536,-7.865, P<0.05). Conclusion The level of 25(OH)D_(3)decreased, the relative richness of Bacteroidetes, Actinomycetes, Proteobacteria, Prevotella, Clostridium and Gordonia in intestinal microflora increased, while the relative richness of Firmicutes, Lactobacillus, Bacteroides, Haemophilus, Veillonella and Eubacterium decreased in RA patients. The level of serum 25(OH)D_(3)was correlated with intestinal microflora distribution in elderly RA patients.