文献类型：期刊文献

中文题名：“疏肝调神”针法调节PTSD大鼠大脑皮层血氧浓度的研究（英文）

英文题名：Study on “liver-soothing and mind-regulating” acupuncture manipulation in regulating the blood-oxygen concentration in cerebral cortex of PTSD rats

作者：张彦峰[1];韩雅迪[1];赵中亭[1];严兴科[1]

第一作者：张彦峰

通信作者：Yan, XK[1]

机构：[1]甘肃中医药大学

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou 730000, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2018

卷号：28

期号：3

起止页码：198

中文期刊名：World Journal of Acupuncture-Moxibustion

外文期刊名：世界针灸杂志（英文版）

收录：Scopus;CSCD:【CSCD2017_2018】；

基金：Supported by Regional fund project of 2014 National Natural Science Foundation of China : 81460744; 2013 Gansu Provincial Natural Science Foundation: 1308RJZA150.

语种：中文

中文关键词：“疏肝调神”针法;fNIRs;血氧浓度

外文关键词：PTSD;＂Liver-soothing and mind-regulating＂;acupuncture manipulation;fNIRs;Blood-oxygen concentration

摘要：目的:探讨"疏肝调神"针法干预创伤后应激障碍(posttraumatic stress disorder,PTSD)的脑功能机制。方法:SD大鼠60只,随机数字法随机分为空白组、模型组、抓取组、帕罗西汀组和针刺组。除空白组外各组大鼠均以电击幽闭法复制PTSD动物模型,模型复制共计7天。三种干预措施组动物分别于应激造模前1 h给予药物灌胃、针刺干预和抓取固定,天为一疗程,连续抓取治疗2个疗程,计12天。造模结束后各治疗组连续治疗5天后利用功能性近红外光谱技术(functional near-infrared spectroscopy,fNIRS)系统采集记录各组大鼠相关脑区组织血红蛋白(Oxy-Hb)、还原血红蛋白(Deoxy-Hb)和总血红蛋白(Total-Hb) 3分钟的浓度变化,进行脑功能评估。结果:通道血氧浓度时间序列比较,模型组、抓取组与空白组有显著性差异(P<0.01,P<0.01),模型组与抓取组未见显著性差异(P>0.05);与抓取组相比,帕罗西汀组、针刺组有显著性差异(P<0.05,P<0.05),帕罗西汀组与针刺组未见显著性差异(P>0.05)。结论:"疏肝调神"针法对PTSD大鼠大脑皮层血氧浓度的有良性调节作用,这可能是"疏肝调神"针法干预PTSD重要脑功能机制。
Objective： To explore the brain function mechanism of ＂liver-soothing and mind-regulating＂ acupuncture manipulation in intervening post-traumatic stress disorder（PTSD）.Methods： According to random number table method, 60 SD rats were randomly divided into blank group, model group, grabbing group, paroxetine group and acupuncture group. Except for the blank group, all the rats were established into PTSD models through electric shock and incarceration, with model replication for 7 days in total. The rats were given gavage, acupuncture intervention and grabbing fixation 1 h before modeling, with 6 days as 1 course of treatment. Grabbing treatment was performed for 2 courses, with 12 days in total. After modeling, functional near-infrared spectroscopy（fNIRS） system was adopted to collect and record the changes of concentration of Oxy-Hb, Deoxy-Hb and Total-Hb during 3 min in related brain regions of rats in each group after treatment for 5 consecutive days, and the brain function was evaluated.Results： According to the comparison of the time series of blood-oxygen concentration, there were significant differences between model group and blank group as well as grabbing group and blank group（P〈0.01, P〈0.01）, no significant difference was found between model group and grabbing group（P〈0.05）; there were significant differences between paroxetine group and grabbing group as well as acupuncture group and grabbing group（P〈0.05, P〈0.05）, no significant difference was found between paroxetine group and acupuncture group（P〈0.05）.Conclusion： ＂Liver-soothing and mind-regulating＂ acupuncture manipulation has a benign regulatory effect on the blood-oxygen concentration in cerebral cortex of PTSD rats, which may be the important brain function mechanism of ＂liver-soothing and mind-regulating＂ acupuncture manipulation in intervening PTSD.