文献类型：期刊文献

英文题名：LncRNA HOXD-AS1 promotes the metastasis of human hepatocellular carcinoma via modulating miR-326/SLC27A4

作者：Ji, Wenbin[1];Wang, Qunfeng[2];Yang, Jian[3]

第一作者：Ji, Wenbin

通信作者：Yang, J[1]

机构：[1]Wenzhou Med Univ, Dept Radiol, Affiliated Taizhou Hosp, Taizhou 317000, Zhejiang, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Inst Integrated Tradit Chinese & Western Med, Lanzhou 730000, Peoples R China;[3]Xi An Jiao Tong Univ, Dept Imaging, Affiliated Hosp 1, Xian 710061, Peoples R China

第一机构：Wenzhou Med Univ, Dept Radiol, Affiliated Taizhou Hosp, Taizhou 317000, Zhejiang, Peoples R China

通信机构：[1]corresponding author), Xi An Jiao Tong Univ, Dept Imaging, Affiliated Hosp 1, Xian 710061, Peoples R China.

年份：2020

卷号：20

期号：1

外文期刊名：CANCER CELL INTERNATIONAL

收录：;Scopus(收录号：2-s2.0-85085099760);WOS:【SCI-EXPANDED(收录号:WOS:000535620800002)】；

语种：英文

外文关键词：HCC; HOXD-AS1; Metastasis; miR-326; SLC27A4

摘要：BackgroundMounting evidences have indicated that long non-coding RNA (lncRNA) HOXD cluster antisense RNA 1 (HOXD-AS1) is dysregulated and participates into the progression of cancers. This study aims to investigate the biological roles and mechanisms of HOXD-AS1 in the metastasis of hepatocellular carcinoma (HCC).MethodsThe quantitative real-time PCR (qPCR) assay was used to assess the level of miR-326 and HOXD-AS1 in HCC tissues and cell lines. The growth of HCC cell was analyzed by using CCK-8 assay and colony formation assay. The migration and invasion of HCC cell were investigated by using wound healing and transwell invasion analysis. The expressions of SLC27A4, N-cadherin and E-cadherin were determined by western blotting. The growth of HCC cell in vivo was assessed by using xenograft model.ResultsHere, we elaborated that HOXD-AS1 was overexpressed in HCC tissues than that in the adjacent normal tissues and the level of HOXD-AS1 was related with the aggressive phenotypes of HCC. Functionally, downregulation of HOXD-AS1 repressed the proliferation, invasion abilities of HCC cell in vitro and the distant metastasis of HCC cell in vivo. Further investigations demonstrated that HOXD-AS1 directly bound with miR-326 and thereby regulated its endogenous target gene, solute carrier family 27 member 4 (SLC27A4).ConclusionsAll these findings indicated that HOXD-AS1-miR-326-SLC27A4 axis participated into the progression of HCC.