文献类型：期刊文献

中文题名：GⅡ.2型诺如病毒P蛋白的表达及组织血型抗原结合模式分析

英文题名：Expression and analysis of histo-blood group antigens binding pattern of GⅡ.2 norovirus P protein

作者：宋皎[1];李涵博[1];丛鑫[2];靳淼[2];李丹地[1];郑贵森[1];段招军[2]

第一作者：宋皎

机构：[1]甘肃中医药大学公共卫生学院,兰州730000;[2]中国疾病预防控制中心病毒病预防控制所,北京102206

第一机构：甘肃中医药大学公共卫生学院

年份：2019

卷号：33

期号：1

起止页码：54

中文期刊名：中华实验和临床病毒学杂志

外文期刊名：Chinese Journal of Experimental and Clinical Virology

收录：CSTPCD;;Scopus;CSCD:【CSCD2019_2020】；PubMed;

语种：中文

中文关键词：诺如病毒;暴发;P蛋白;组织血型抗原

外文关键词：Norovirus;Outbreaks;P protein;Histo-blood group antigens

摘要：目的2016年冬季,GⅡ.2型诺如病毒(norovirus,NoV)在我国暴发流行,为了阐明其流行机制,本文对其衣壳蛋白P区与组织血型抗原(histo-blood group antigens,HBGAs)结合特征进行研究。方法选取2016年底北京市暴发的GⅡ.2型ZTX株为研究对象,构建重组的原核表达质粒,表达纯化得到病毒P蛋白,通过唾液和寡糖结合实验对其与HBGAs的结合特征进行研究。结果成功获得可溶性P蛋白,P蛋白与A型,B型,AB型唾液均结合。结论明确了北京暴发的GⅡ.2型NoV的唾液的结合特征,为其致病机制及防控措施提供了一定的基础和依据。
Objective During the 2016 winter season,GⅡ.2 norovirus (NoV) suddenly emerged in China.To elucidate its mechanism of epidemic,this study focused on characteristics of binding between the P protein of capsid and histo-blood group antigens (HBGAs).Methods The research object was GⅡ.2 ZTX strain which had an outbreaks by the end of 2016 in Beijing.Recombinant prokaryotic expression plasmid was constructed,and the expression of virus P protein was determined and purified.The P protein characteristics of binding to HBGAs was studied through saliva and oligosaccharide binding experiments.Results Soluble P protein was successfully obtained,and combined with type A,B,AB saliva.Conclusions The result illuminate the combination with new outbreaks of NoV and salivary types,which provided a basis for its pathogenic mechanism and prevention and control measures.