文献类型：期刊文献

英文题名：Endophytic Bacillus pumilus G5 with silicon alleviates drought stress and enhances glycyrrhizic acid in Glycyrrhiza uralensis Fisch. via ABA and JA signaling

作者：Wang, Yufeng[1,2];Lang, Duoyong[3];Cui, Gaochang[1,4,5];Xu, Zhanchao[1,6];Zhang, Xiaojia[1,2];Huang, Yufang[7];Zhang, Wenjin[1,2];Zhang, Xinhui[1,2]

第一作者：Wang, Yufeng

通信作者：Zhang, WJ[1];Zhang, XH[1]

机构：[1]Ningxia Med Univ, Coll Pharm, Yinchuan 750004, Peoples R China;[2]Minist Educ, Ningxia Engn & Technol Res Ctr Reg Characterizist, Ningxia Collaborat Innovat Ctr Reg Characterizist, Key Lab Protect Dev & Utilizat Med Resources Liupa, Yinchuan 750004, Peoples R China;[3]Ningxia Med Univ, Coll Basic Med, Yinchuan 750004, Peoples R China;[4]Shaanxi Univ Chinese Med, Coll Pharm, Xianyang 712046, Peoples R China;[5]Shaanxi Univ Chinese Med, Shaanxi Qinling Applicat Dev & Engn Ctr Chinese He, Xianyang 712046, Peoples R China;[6]Northwest Univ First Hosp, Xian 710043, Shaanxi, Peoples R China;[7]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China

第一机构：Ningxia Med Univ, Coll Pharm, Yinchuan 750004, Peoples R China

通信机构：[1]corresponding author), Ningxia Med Univ, Coll Pharm, Yinchuan 750004, Peoples R China.

年份：2026

卷号：243

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20261420419293);Scopus(收录号：2-s2.0-105034596532);WOS:【SCI-EXPANDED(收录号:WOS:001730528200001)】；

基金：This work is grateful for the financial support provided by the project of the National Natural Science Foundation of China (82160724) , the Ningxia Natural Science Foundation (2024AAC03272) , and University-Level Special Talent Initiation Project (XT2023024) .

语种：英文

外文关键词：Drought stress; G5 interacts with silicon; Hormone signal; Active ingredients; Physiological and gene attributes

摘要：Drought stress is a major challenge for global agriculture, impacting the production of medicinal plants. Plant growth-promoting bacteria (PGPB) and silicon (Si), an environmentally friendly element, are both known to support plant adaptation to drought and enhance the accumulation of bioactive metabolites. In this study, we investigated how Bacillus pumilus G5 (G5) and Si regulate hormone biosynthesis, signal transduction, and glycyrrhizic acid formation in Glycyrrhiza uralensis under drought conditions. Physiological measurements combined with transcriptomic profiling were used to clarify the relationship between hormone pathways and glycyrrhizic acid production. Drought treatment decreased glycyrrhizic acid levels by suppressing the expression of HMGR, HDR, and FPPS. In contrast, the combined G5 + Si treatment markedly improved glycyrrhizic acid accumulation by stimulating the enzymatic activities of FPPS, SQS, and UGT. Although G5 + Si enhanced Crtz and NCED activities, it did not change ABA concentrations in drought-stressed plants, indicating that this treatment maintains ABA homeostasis. Instead, it accelerates the turnover between ABA biosynthesis and degradation, thereby activating PYR/PYL and SnRK2 and improving drought resistance. Furthermore, G5 + Si mitigated drought damage by promoting JA signaling and up-regulating MYC2, ultimately increasing both drought tolerance and glycyrrhizic acid accumulation. These findings emphasise the strong synergy between G5 and Si in supporting stress mitigation and enhancing the medicinal quality of G. uralensis.