文献类型：期刊文献

英文题名：Integrated physiological characterisation and transcriptomics reveals drought tolerance differences between two cultivars of A. sinensis at seedling stage

作者：Zhu, Tiantian[1,2,3,4];Liu, Tianle[1];Kang, Shuqi[1];Zhang, Jing[1];Zhang, Shuai[1];Yang, Baimei[1];Ma, Xiaohui[1,2,3,4];Guo, Liu[5];Li, Mengfei[6];Jin, Ling[1,2,3,4]

第一作者：Zhu, Tiantian;朱田田

通信作者：Zhu, TT[1];Jin, L[1];Zhu, TT[2];Jin, L[2];Zhu, TT[3];Jin, L[3];Zhu, TT[4];Jin, L[4]

机构：[1]Gansu Univ Tradit Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[2]Northwest Collaborat Innovat Ctr Traditiona Chines, Lanzhou 730000, Gansu, Peoples R China;[3]MOE PRC, Lanzhou 730000, Gansu, Peoples R China;[4]Engn Res Ctr Evaluat Protect & Utilizat Rare Tradi, Lanzhou 730000, Gansu, Peoples R China;[5]Longxi Qizheng Med Mat Co Ltd, Longxi 748112, Gansu, Peoples R China;[6]Gansu Agr Univ, State Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Tradit Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[2]corresponding author), Northwest Collaborat Innovat Ctr Traditiona Chines, Lanzhou 730000, Gansu, Peoples R China;[3]corresponding author), MOE PRC, Lanzhou 730000, Gansu, Peoples R China;[4]corresponding author), Engn Res Ctr Evaluat Protect & Utilizat Rare Tradi, Lanzhou 730000, Gansu, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2025

卷号：52

期号：1

外文期刊名：MOLECULAR BIOLOGY REPORTS

收录：;Scopus(收录号：2-s2.0-86000084746);WOS:【SCI-EXPANDED(收录号:WOS:001439185400001)】；

基金：This research was funded by Project supported by the Foundation for Young Doctoral Fellowships for Universities of Gansu Province [Grant Number 2023QB-094], Gansu Province Science and Technology Major Special Project [Grant Number 23ZDFA013-1], Double First-Class Major Scientific Research Project of the Gansu Provincial Department of Education [Grant Number GSSYLXM-05], the Strategic Research and Consulting Project of the Chinese Academy of Engineering [Grant Number GS2021ZDA06], and Gansu Province Science and Technology Program [Grant Number 20JR5RA182].

语种：英文

外文关键词：Angelica sinensis (Oliv.) Diels; Drought stress; Varietal differences; Physiological characteristics; Transcriptome analysis; Drought tolerance

摘要：BackgroundDrought can adversely affect the growth and development of Angelica sinensis (Oliv.) Diels seedlings. M1 and M2 are two cultivars of A. sinensis with distinct phenotypic traits, and they may exhibit different stress resistances. This study compares the growth, physiological characteristics, and transcriptome data of two cultivars of A. sinensis seedlings to explore their differences in drought tolerance and the underlying molecular mechanisms.Methods and resultsWe treated seedlings of M1 and M2 using the natural drought method in pots and compared their growth, physiological characteristics, and transcriptomic data. Under drought stress, M2 showed less phenotypic changes and higher RWC of leaves, photosynthetic parameters, and antioxidant enzyme activities. By analyzing RNA-seq data, 13,941 and 9913 DEGs were identified from M1 and M2. These DEGs are enriched in important pathways related to plant responses to drought stress, including photosynthesis (LHC, HEMA, PAO), antioxidant defense (APX, GPX, EBF1/2), and secondary metabolites (PAL, 4CL, CAD). A thorough analysis of the significant DEGs between the two cultivars of A. sinensis revealed that the expression levels of these DEGs were generally consistent with the physiological changes observed under drought stress. In addition, key transcription factors such as WRKY6, PAT1, and SCL13 were screened through WGCNA.ConclusionsM2 seedlings showed greater drought tolerance compared to M1. The research results not only provide insights into the molecular mechanisms of A. sinensis in response to drought stress, but also offer a reference for the evaluation of A. sinensis germplasm resources and the selection and breeding of drought-tolerant cultivars.