文献类型：期刊文献

中文题名：痛泻要方对溃疡性结肠炎大鼠下丘脑中IL-6,IL-6R表达的影响

英文题名：Effect of Tongxie Yaofang on IL-6,IL-6R mRNA and Protein Expressions of Hypothalamus Tissues in Rats with Experimental Ulcerative Colitis

作者：李婷[1];朱向东[1];杨意[1];翟艳会[1]

第一作者：李婷

机构：[1]甘肃中医药大学,兰州730000

第一机构：甘肃中医药大学

年份：2017

卷号：23

期号：11

起止页码：103

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2017_2018】；

基金：国家自然科学基金项目(81460696)

语种：中文

中文关键词：痛泻要方;溃疡性结肠炎;白细胞介素-6(IL-6);白细胞介素-6受体(IL-6R);基因表达;蛋白表达

外文关键词：Tongxie Yaofang; ulcerative colitis; interleukin-6 （IL-6）; interleukin-6 receptors （IL-6R）; gene expression; protein expression

摘要：目的:观察痛泻要方对溃疡性结肠炎(ulcerative colitis,UC)大鼠下丘脑中白细胞介素-6(IL-6)和白细胞介素-6受体(IL-6R)基因和蛋白表达的影响。方法:将SPF级Wistar大鼠运用复合方法进行溃疡性结肠炎造模后,随机分为5组:模型组,痛泻要方低、中、高剂量(2.75,5.5,11 g·kg^(-1))组,美沙拉嗪(5-ASA)组。药物处理大鼠21 d后麻醉处死,肉眼观察结肠大体形态损伤并进行评分。分离各组大鼠血清,酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测血清中IL-6含量,分离大鼠下丘脑组织并提取总RNA后,实时荧光定量PCR(Real-time polymerase chain reaction,Real-time PCR)法检测IL-6和IL-6R基因表达,免疫组织化学染色法检测下丘脑组织IL-6和IL-6R的分布及蛋白表达量。结果:ELISA结果显示,与空白组比较,模型组大鼠血清中IL-6含量明显升高,经过药物处理后,药物组血清中IL-6含量均有所下降,其中以痛泻要方高剂量组效果最为明显(P<0.05)。Real-time PCR结果表明,模型组大鼠下丘脑组织中IL-6和IL-6R基因表达量明显高于空白组(P<0.05),经药物治疗后,药物组IL-6和IL-6R基因表达均有所降低,其中以5-ASA组和痛泻要方高剂量组降低最为明显(P<0.05)。免疫组织化学染色结果表明,与空白组比较,溃疡性结肠炎大鼠IL-6和IL-6R蛋白表达量明显增加(P<0.05),药物处理可以降低大鼠下丘脑IL-6和IL-6R蛋白表达量,其中以5-ASA组和痛泻要方高剂量组降低最为明显(P<0.05)。结论:痛泻要方对2,4,6三硝基苯磺酸(2,4,6-trinitrobenzene sulfonic acid,TNBS)/乙醇法UC大鼠下丘脑组织中IL-6和IL-6R基因和蛋白的表达具有明显抑制作用,提示痛泻要方治疗溃疡性结肠炎的机制可能与抑制下丘脑中IL-6/IL-6R信号通路的异常活化有关。
Objective： To explore the curative effect of Tongxie Yaofang on ulcerative colitis （UC） in rat hypothalamus cytokine interleukin-6 （IL-6） and interleukin-6 receptors （IL-6R） gene and protein expressions. Method： After the ulcerative colitis model was established by the composite method. SPF Wistar rats were randomly divided into 5 groups： model group, low, middle, high-dose Tongxie Yaofang groups （2.75, 5.5, 11 g＇kg-＇ ） , and 5-ASA group. After modeling, rats were given drugs for 21 days. Then, colonic mucosa damage was observed. The levels of IL-6 in the serum tissues were detected by enzyme linked immunosorbent assay （ELISA）. The proteins expressions of IL-6 and IL-6R in rat hypothalamus tissues were detected by immunohistochemistry, and their gene expressions were measured by Real-time PCR method. Result： ELISA test results show that compared with control group, model group showed significant increase in content of IL-6 in serum of rats. After drug treatment, the serum IL-6 in drug group were decreased, with the most obvious effect in high- dose Tongxie Yaofang group （P 〈 0.05）. Real-time PCR results showed that model group＇s hypothalamic IL-6 and IL-6R gene expressions were higher than those of blank group （P 〈 0.05）. After treatment with drugs, drug group＇s IL-6 and IL-6R gene expressions were reduced, and 5-ASA and high-dose Tongxie Yaofang groups were superior to the other groups （P 〈 0.05）. Immunohistochemical staining results showed that compared with control group, [L-6 and IL-6R protein expressions of ulcerative colitis rats increased significantly （P 〈 0.05）, drug treatment can reduce their hypothalamic IL-6 and IL-6R protein expressions, and 5-ASA and high-dose Tongxie Yaofang groups were superior to the other groups （P 〈 0.05）. Conclusion： Tongxie Yaofang can obviously inhibit IL-6 and IL-6R gene and protein expressions in hypothalamic tissues of the rat UC model by TNBS/ethanol method, suggesting Tongxie Yaofang＇s mechanism in the treatment of ulcerative colitis may be correlated with inhibition of abnormal activation of IL-6/IL-6R signaling pathway in hypothalamus.