文献类型：期刊文献

中文题名：两种芽孢杆菌配施纳米铁对成药期党参根际土壤养分及微生物类群的影响

英文题名：Bacillus isolates coupled with nanoscale zero-valent iron influenced rhizosphere soil nutrients and microbial community during the medication period of Codonopsis pilosula

作者：王丹[1,2];赵毅[3];宋婕[2];陈玉坤[2];孟令煜[1];吴志国[3];张小朋[4];祝英[2];王引权[1,5]

第一作者：王丹

机构：[1]甘肃中医药大学药学院,兰州730000;[2]甘肃省科学院生物研究所,甘肃省微生物资源开发利用重点实验室,兰州730000;[3]甘肃省科学院纳米应用技术研究室,兰州730000;[4]华中农业大学生命科学与技术学院,微生物农药国家工程研究中心,农业微生物国家重点实验室,武汉430000;[5]西北中藏药省部共建协同创新中心,甘肃省中药质量与标准研究重点实验室培育基地,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2023

卷号：29

期号：5

起止页码：1261

中文期刊名：应用与环境生物学报

外文期刊名：Chinese Journal of Applied and Environmental Biology

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：甘肃省科学院重大专项(2023zdzx-01);国家自然科学基金项目(41967018);甘肃省教育厅双一流重大科研项目(GSSYLXM-05);西北中藏药省部共建协同创新中心开放基金项目(Xbzzy-2022-01);兰州市科技局科技研发项目(2021-1-158);甘肃省科技厅重大专项(21ZD4NA016);甘肃省科技厅重点项目(21YF5FH211);甘肃省委组织部陇原青年创新创业人才个人项目(2019)资助

语种：中文

中文关键词：党参;微生物菌剂;纳米铁;土壤养分;可培养微生物类群;土壤酶活性

外文关键词：Codonopsis pilosula;microbial fertilizer;nanoscale zero-valent iron;soil nutrient;culturable microbial population;soil enzyme activity

摘要：研究芽孢杆菌配施纳米铁对成药期党参根际土壤养分和可培养微生物类群数的调节作用,可为党参生态种植提供技术支撑.田间试验于党参种苗移栽时分别用有效活菌数为7.41亿/mL的枯草芽孢杆菌农用微生物菌剂和6.92亿/mL的蜡样芽孢杆菌复合微生物肥料浸苗处理,在出苗后1个月叶面喷施浓度为10mg/L的纳米铁.于叶盛期测定地上部分生物量(株高、茎粗、叶宽等);采集土壤样品进行土壤养分和微生物类群分析.结果显示:枯草芽孢杆菌配施纳米铁处理(KF)和蜡样芽孢杆菌配施纳米铁处理(LF)均促能促进党参营养生长.其中KF和LF处理对党参株高和冠幅较对照(CK)分别提高了76.49%、69.43%和121.18%、93.49%;不同处理显著影响了根际土壤电导率(EC),有效钾在根际土的积累和转化利用(P<0.05),同时也改变根际土细菌、真菌、放线菌、氨化细菌和固氮菌等可培养微生物的类群数量;不同处理的党参土壤过氧化氢酶(S-CAT)、土壤脲酶(S-UE)和土壤蔗糖酶(S-SC)活性均显著高于CK,其中KF和LF处理的党参S-CAT活性较对照显著提高了121.81%和159.44%,且KF处理的党参S-UE活性较对照显著提高了119.52%.不同处理条件下,根际土壤酶活性与微生物和养分的相关性分析结果显示,不同处理不但改变土壤酶活性与可培养微生物类群数量和根际土养分的交互关系,而且可培养微生物类群的改变与根际土养分的积累、转化利用存在一定的协同效应.本研究表明两种芽孢杆菌微生物肥料配施纳米铁通过影响根际微生物类群数量和功能,提高了党参土壤酶活性、改善了根际土壤养分周转和植物的吸收利用,进而促进了党参生长.
Regulation of soil microbial and nutrient metabolic functions of two types of Bacillus bacteria coupled with nanoscale zero-valent iron(nZVI)during the medication period of Codonopsis pilosula was explored.The seedlings were treated with 7.41×109 CFU/mL B.subtilis and 6.92×109 CFU/mL B.cereus agents and sprayed with 10 mg/L nZVI 1 month after their emergence.Aboveground biomass(plant height,stem diameter,and leaf width)at the leaf bloom was measured,and soil samples were collected for soil nutrient and microorganism analyses.The results showed that both Bacillus species coupled with nZVI promoted the growth of C.pilosula.B.subtilis combined with nano-iron(KF)and B.cereus combined with nano-iron(LF)effectively increased the height and breadth of C.pilosula by 76.49%and 69.43%and 121.18%and 93.49%,respectively,compared with the control(CK)treatment.Different treatments significantly affected the accumulation and conversion of electrical conductivity and available potassium in the rhizosphere soil(P<0.05)and altered the number of soil microbial populations,such as rhizosphere soil bacteria,fungi,actinomycetes,ammonifiers,and azotobacter.The S-CAT,S-UE,and S-SC activities of C.pilosula were significantly higher upon treatment with the microbial agents,nZVI,and a combination of both than under the CK treatment.The S-CAT activities of C.pilosula were significantly increased by 121.81%and 159.44%upon treatment with KF and LF,respectively,compared with CK treatment,and the S-UE activity upon KF treatment was significantly higher by 119.52%than that upon CK treatment.Correlation analysis results of rhizosphere soil enzyme activities with microorganisms and nutrients under different treatments not only revealed that treatment with the microbial agent,nZVI,or both changed the correlation of soil enzyme activity with microorganism population and rhizosphere soil nutrients,but also revealed that the changing microorganism population had some synergistic effects with soil nutrient turnover and plant absorption and utilization.In conclusion,the application of two Bacillus agents coupled with nZVI improved the nutrient content of rhizosphere soil as well as plant uptake and utilization by affecting the population and function of rhizosphere microorganisms to improve soil enzyme activity,thus boosting the biomass of C.pilosula.Microbial agents and nanofertilizers show great potential for application to the cultivation of C.pilosula.