文献类型：期刊文献

中文题名：胃癌相关易感基因的生物信息学分析

英文题名：Bioinformatics analysis of gastric cancer-related susceptibility genes

作者：李玲[1];苏韫[1];刘永琦[1,2];骆亚莉[1];张丽英[1];王凤梅[1];任春贞[1];卢志伟[1];柳峰峰[1]

第一作者：李玲

机构：[1]甘肃中医药大学,甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,兰州730000;[2]敦煌医学与转化省部共建教育部重点实验室,兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2018

卷号：33

期号：6

起止页码：2390

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金项目(No.81660744)

语种：中文

中文关键词：胃癌;易感基因;生物信息

外文关键词：Gastric cancer;Susceptibility gene;Bioinformatics

摘要：目的:归纳分析胃癌易感基因的相关文献,并进行生物信息学分析,为进一步的研究提供参考依据。方法:检索Pub Med、Embase、中国知网数据库、万方数据库2006年1月1日至2015年12月31日发表的文献,摘录胃癌易感基因并进行GO分类、KEGG通路分析、在线STRING 9.05软件构建蛋白-蛋白相互作用网络图、Cytoscape2.8.2软件可视化分析。结果:共检索有效文献405篇,摘录胃癌相关易感基因188个,不同分级的GO分类201种,KEGG通路13种,这些基因主要与细胞损伤修复、免疫反应、细胞周期调节、炎性反应、DNA损伤修复、凋亡和抗原递呈等有关。KEGG通路主要是参与细胞因子-细胞因子受体相互作用、细胞凋亡、Toll样受体信号通路、谷胱甘肽代谢、MAPK信号转导通路、脂肪酸代谢等。其中178个可翻译成蛋白的蛋白与蛋白相互作用网络图,提示这些基因的表达产物大部分存在密切的相互关系。进一步可视化及量化,筛选出TP53、VEGF-α、SOD2、TNF、STAT3、BRCA1、PTGS2、PTEN、TGF-β1 9个基因蛋白网络节点度数超过30。结论:TP53、VEGF-α、SOD2、TNF、STAT3、BRCA1、PTGS2、PTEN、TGF-β1 9个基因可能为胃癌易感关键基因。
Objective： To summarize and analyze the relevant literature on susceptibility genes of gastric cancer, and to conduct bioinformatics analysis, so as to provide a reference for further research. Methods： The literature published in the Pub Med, Embase, CNKI and Wan Fang databases from January 1, 2006 to December 31, 2015 were retrieved. Extract gastric cancer easy feeling gene and GO classification, KEGG pathway analysis, used online STRING 9.05 software to construct protein protein interaction network diagram, Cytoscape 2.8.2 software for visual analysis. Results： A total of 405 effective documents were retrieved, 188 of the susceptibility genes related to gastric cancer were extracted. There were 201 different classifications of GO, and 13 species of KEGG pathway. These genes were mainly related to cell damage repair, immune response, cell cycle regulation, inflammatory response, DNA damage repair, apoptosis, and antigen presentation. In the KEGG pathway, it was mainly involved in cytokine-cytokine receptor interaction, cell apoptosis, Toll like receptor signaling pathway, glutathione metabolism, MAPK signal transduction pathway, fatty acid metabolism and so on. A network diagram of 178 translatable proteins and proteins, which suggested that most of these genes were closely related to the expression products. Further visualization and quantitative analysis, 9 genes and more than 30 network nodes were selected, such as TP53, VEGF-α, SOD2, TNF, STAT3, BRCA1, PTGS2, PTEN, and TGF-β1. Conclusion： TP53, VEGF-α, SOD2, TNF, STAT3, BRCA1, PTGS2, PTEN, TGF-β1 genes may be the key genes of gastric cancer susceptibility.