文献类型：期刊文献

中文题名：藏药蕨麻对实验性酒精肝损伤小鼠的保护作用研究

英文题名：Protective effect of Potentilla anserina on ethanol-induced liver damage in mice

作者：罗慧英[1];黄亚红[1];朱丽娟[1];王丽娟[1]

第一作者：罗慧英

机构：[1]甘肃中医学院药理教研室

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2014

卷号：19

期号：10

起止页码：1107

中文期刊名：中国临床药理学与治疗学

外文期刊名：Chinese Journal of Clinical Pharmacology and Therapeutics

收录：CSTPCD;;CSCD:【CSCD2013_2014】；

基金：甘肃省教育厅高校科研项目(2013A-078)

语种：中文

中文关键词：蕨麻;酒精性肝损伤;肝功能;脂质过氧化;自由基

外文关键词：Potentilla anserina; ethanol-induced liver damage; liver function; lipid overoxidation reactions; free radical

摘要：目的:探讨藏药蕨麻对实验性酒精肝损伤小鼠的保护作用。方法:50只小鼠随机分为5组:空白组,模型组,小、中、大剂量蕨麻组,50%酒精灌胃复制实验性酒精肝损伤模型,蕨麻每日2次(50、100、200g/kg),酒精每日1次(10mL/kg),连续4周。小鼠最后1次给药4h后,称重,眶静脉丛取血,离心取血清,全自动生化仪检测肝功指标[AST、ALT、碱性磷酸酶(ALP)];摘取肝脏称重,计算肝脏指数;剪取适量肝脏制成匀浆,检测肝脏组织中TG、TC、丙二醛(MDA)、脂质过氧化物(LPO)含量以及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性;剩余肝脏10%甲醛固定后进行病理学检测。结果:小鼠酒精肝损伤后肝脏指数、AST、ALT、ALP明显升高(P<0.05);肝脏组织中TG、TC、MDA、LPO含量明显高于空白组(P<0.05);SOD、GSH-Px、CAT活性明显降低(P<0.05);病理学检测发现肝脏组织出现炎性浸润和脂肪空泡。蕨麻可明显改善这些现象,表现为降低肝脏指数和肝功指标(P<0.05),减少肝脏组织中TG、TC、MDA、LPO含量(P<0.05),增强SOD、GSH-Px和CAT活性(P<0.05),减少肝脏组织炎性浸润和脂肪空泡的产生。其中大剂量蕨麻的作用更加明显。结论:蕨麻可能通过减少自由基的产生,增强对自由基及其代谢产物的清除能力,从而抑制脂质过氧化,起到对酒精性肝损伤的保护作用。
AIM：To study the protection of Potentilla anserina on ethanol-induced liver damage in mice.METHODS：50 mice were divided into 5groups in random,and administered by gavage for 4weeks.Potentillaanserina was given twice a day,ethanol once a day.When time limit arrived,mice were weighted,serum was afforded to detect liver function index,liver homogenate were prepared for biochemical detections,liver remained were fixed for pathological investigation.RESULTS：When mice damaged by ethanol,liver index,liver function index,contents of TG,TC,MDA and LPO increased distinctly（P〈0.05）,activities of SOD,GSHPx and CAT decreased at the same time（P〈0.05）.Inflammatory infiltration and flat vacuoles were observed.Potentilla anserina ameliorated these changes evidently;the effects were dose-dependent. CONCLUSION： Protective effects of Potentilla anserina on ethanol-induced liver damage in mice were associated with antioxidation,enhancement of clearance of free radicals and metabolites,and inhibition of lipid overoxidation reactions.