文献类型：期刊文献

英文题名：Polysaccharide extracted from Potentilla anserina L ameliorate acute hypobaric hypoxia-induced brain impairment in rats

作者：Shi, Jipeng[1,2,3];Wang, Jinhui[4];Zhang, Ji[2,3];Li, Xiaolin[1,5];Tian, Xiuyu[1,6];Wang, Weigang[1,5];Wang, Peng[1,5];Li, Maoxing[1,5,6]

第一作者：Shi, Jipeng

通信作者：Li, MX[1]

机构：[1]940th Hosp Joint Logist Support Force Chinese PLA, Dept Pharm, Key Lab Prevent & Treatment Injury Plateau PLA, Lanzhou 730050, Gansu, Peoples R China;[2]Northwest Normal Univ, Dept Biochem, Coll Life Sci, Lanzhou, Peoples R China;[3]Bioact Prod Engn Res Ctr Gansu Distinct Plants, Dept Biochem, Lanzhou, Peoples R China;[4]Gansu Univ Chinese Med, Affiliated Hosp, Dept Pharm, Lanzhou, Gansu, Peoples R China;[5]Gansu Univ Chinese Med, Dept Pharm, Coll Pharm, Lanzhou, Peoples R China;[6]Lanzhou Univ, Dept Pharm, Coll Pharm, Lanzhou, Peoples R China

第一机构：940th Hosp Joint Logist Support Force Chinese PLA, Dept Pharm, Key Lab Prevent & Treatment Injury Plateau PLA, Lanzhou 730050, Gansu, Peoples R China

通信机构：[1]corresponding author), 940th Hosp Joint Logist Support Force Chinese PLA, Dept Pharm, Key Lab Prevent & Treatment Injury Plateau PLA, Lanzhou 730050, Gansu, Peoples R China.

年份：2020

卷号：34

期号：9

起止页码：2397

外文期刊名：PHYTOTHERAPY RESEARCH

收录：;Scopus(收录号：2-s2.0-85083462847);WOS:【SCI-EXPANDED(收录号:WOS:000526157300001)】；

基金：Funding for Basic Scientific Research of China's Central Universities, Grant/Award Number: 31920200010; National Natural Science Foundation of China, Grant/Award Number: 51873175; Natural Science Foundation of Gansu Province, Grant/Award Number: 17JR5RA332; Special Foundation Projects for Guiding Technological Innovation and Development of Gansu Province, Grant/Award Number: 2019ZX-05; Top Project of Medical Technology for Youth of PLA, Grant/Award Number: 16QNP055; University Research and Innovation Team Project of Gansu Province, Grant/Award Number: 2018C-04

语种：英文

外文关键词：acute hypobaric hypoxia; high altitude cerebral edema; inflammatory response; oxidative stress; Potentilla anserina L polysaccharide

摘要：High altitude cerebral edema (HACE) is a high altitude malady caused by acute hypobaric hypoxia (AHH), in which pathogenesis is associated with oxidative stress and inflammatory cytokines. Potentilla anserina L is mainly distributed in Tibetan Plateau, and its polysaccharide possesses many physiological and pharmacological properties. In the present study, the protective effect and potential treatment mechanism of Potentilla anserina L polysaccharide (PAP) in HACE were explored. First, we measured the brain water content and observed the pathological changes in brain tissues, furthermore, malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), and glutathione (GSH) were evaluated by kits. Finally, the protein contents and mRNA expressions of pro-inflammatory (IL-1 beta, IL-6, TNF-alpha, vascular endothelial cell growth factor [VEGF], NF-kappa B, and hypoxia inducible factor-1 alpha [HIF-1 alpha]) were detected by ELISA kits, RT-PCR, and western blotting. The results demonstrated that PAP reduced the brain water content, alleviated brain tissue injury, reduce the levels of MDA and NO, and increased the activity of SOD and GSH level. In addition, PAP blocking the NF-kappa B and HIF-1 alpha signaling pathway activation inhibited the generation of downstream pro-inflammatory cytokines (IL-1 beta, IL-6, TNF-alpha, and VEGF). Therefore, PAP has a potential to treat and prevent of HACE by suppression of oxidative stress and inflammatory response.