文献类型：期刊文献

英文题名：???????In vitro bioactive metabolite production and plant regeneration of medicinal plant Angelica sinensis

作者：Huang, Tianmiao[1];Liu, Di[1];Cui, Xiuwen[1];Li, Meiling[1];Jin, Ling[2];Pare, Paul W.[3];Li, Mengfei[1];Wei, Jianhe[4]

第一作者：Huang, Tianmiao

通信作者：Li, MF[1];Wei, JH[2]

机构：[1]Gansu Agr Univ, State Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China;[2]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[3]Texas Tech Univ, Dept Chem & Biochem, Lubbock, TX 79409 USA;[4]Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, Beijing 100193, Peoples R China

第一机构：Gansu Agr Univ, State Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China

通信机构：[1]corresponding author), Gansu Agr Univ, State Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China;[2]corresponding author), Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, Beijing 100193, Peoples R China.

年份：2023

卷号：194

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000964493800001)】；

基金：This research was funded by the State Key Laboratory of Aridland Crop Science/Gansu Agricultural University Key(GSCS-2021-Z03), National Natural Science Foundation of China (32160083), Assurance Project of Ecological Planting and Quality of Daodi Herbs (202103003),and earmarked fund for CARS (CARS-21).

语种：英文

外文关键词：Angelica sinensis; Histomorphological structure; Suspension cell culture; Bioactive metabolite; Plant regeneration; Culture condition

摘要：The root of Angelica sinensis (Oliv.) Diels is a traditional Chinese medicine (TCM) widely used for the cardiocerebrovascular, anti-inflammatory, and anti-cancer agents, etc. Currently, the root yield is mainly limited by narrow geographical distribution, early bolting and flowering (EBF), continuous cropping obstacle, and germplasm degeneration. While two ancillary strategies to produce bioactive metabolites by bioreactor and innovate new germplasms by genome editing have not been conducted. Here is reported embryogenic callus induction, histomorphological structure identification, suspension cell culture, bioactive metabolite determination, shoot bud and root differentiation, and plantlet acclimatization at different treatments such as explants, hormones, and culture conditions. The embryogenic calli can be effectively induced (99.0%) from the root of aseptic seedlings by a combination of 2.0 mg/L IBA and 0.2 mg/L KT, and embryogenic cells including: three-cell, globular-, heart-, torpedo-, and cotyledon-shaped embryos were observed. The maximum growth rate, bioactive metabolites contents (i.e., ferulic acid, soluble sugar, flavonoids, and phenolic), and antioxidant capacities were observed at 9-12 d with inoculum density 40 g/L, 22 celcius and 16/8 h light/dark. Robust plantlets can be obtained within 180 d after successive shoot bud and root differentiation as well as plantlet acclimation. Based on the strategies for the satisfaction of root demand, an effective and complete scheme is proposed for producing bioactive metabolites and regenerating plant of A. sinensis.