文献类型：期刊文献

英文题名：Integrated multi-omics analysis reveals the mechanisms of naringin in ameliorating high-fat diet-induced metabolic dysfunction-associated steatotic liver disease

作者：Sun, Wenping[1];Xue, Na[2];Zhang, Qiang[3]

第一作者：Sun, Wenping

通信作者：Zhang, Q[1]

机构：[1]Gansu Prov Canc Hosp, Surg Hepatobiliary & Pancreat Canc, Lanzhou, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Sch Basic Med, Lanzhou, Gansu, Peoples R China;[3]Gansu Prov Canc Hosp, Urol Surg, Lanzhou, Gansu, Peoples R China

第一机构：Gansu Prov Canc Hosp, Surg Hepatobiliary & Pancreat Canc, Lanzhou, Gansu, Peoples R China

通信机构：[1]corresponding author), Gansu Prov Canc Hosp, Urol Surg, Lanzhou, Gansu, Peoples R China.

年份：2025

卷号：12

外文期刊名：FRONTIERS IN NUTRITION

收录：;Scopus(收录号：2-s2.0-105020823494);WOS:【SCI-EXPANDED(收录号:WOS:001606275700001)】；

语种：英文

外文关键词：naringin; MASLD; lipid metabolism; gut microbiota; network pharmacology

摘要：Introduction Naringin (Nar), the predominant flavonoid in citrus fruits, shows therapeutic potential against metabolic dysfunction-associated steatotic liver disease (MASLD). However, its underlying mechanisms remain largely elusive.Methods In this study, we investigated the efficacy and underlying mechanisms of Nar in a mouse model of high-fat diet (HFD)-induced MASLD using integrated analyses of network pharmacology, molecular docking, hepatic lipidomics, and gut microbiota.Results Treatment with Nar markedly ameliorated MASLD phenotypes, as evidenced by reduced body and liver weights, lower hepatic triglycerides (TGs), and improved serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. Network pharmacology analysis revealed that Nar targets associated with MASLD are primarily enriched in proteins such as SRC, AKT1, STAT3, FOS, ESR1, and NFKB1, which exert their effects through the PI3K-AKT signaling pathway. Molecular docking simulations further elucidated the interaction mechanisms. Lipidomic analysis revealed that Nar restored hepatic lipid homeostasis, significantly decreasing levels of TGs and diglycerides (DGs), with 20 differentially abundant lipid species identified as potential biomarkers. Additionally, Nar profoundly altered the gut microbial community, promoting the enrichment of beneficial genera including Oscillibacter, Allisonella, and Flavonifractor.Discussion Our findings indicate that Nar prevents MASLD by harmonizing hepatic lipid metabolism and modulating the gut microbiome, providing a multifaceted mechanistic insight into its therapeutic potential.