文献类型：期刊文献

英文题名：Combination of Metabolomic Analysis and Transcriptomic Analysis Reveals Differential Mechanism of Phenylpropanoid Biosynthesis and Flavonoid Biosynthesis in Wild and Cultivated Forms of Angelica sinensis

作者：Wang, Yuanyuan[1];Zhang, Jialing[1];Chen, Yiyang[2];Liu, Juanjuan[3];Li, Ke[1,4];Jin, Ling[1,4,5,6]

第一作者：汪元元;王圆圆;王媛媛

通信作者：Jin, L[1];Jin, L[2];Jin, L[3];Jin, L[4]

机构：[1]Gansu Univ Tradit Chinese Med, Sch Pharm, Lanzhou 730101, Peoples R China;[2]Nanjing Univ Chinese Med, Sch Pharm, Nanjing 210023, Peoples R China;[3]China Acad Chinese Med Sci, Inst Chinese Mat Med, Resource Ctr Chinese Mat Med, Beijing 100700, Peoples R China;[4]Gansu Pharmaceut Ind Innovat Res Inst, Lanzhou 730000, Peoples R China;[5]Northwest Collaborat Innovat Ctr Tradit Chinese Me, Lanzhou 730000, Peoples R China;[6]MOE PRC, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Tradit Chinese Med, Sch Pharm, Lanzhou 730101, Peoples R China;[2]corresponding author), Gansu Pharmaceut Ind Innovat Res Inst, Lanzhou 730000, Peoples R China;[3]corresponding author), Northwest Collaborat Innovat Ctr Tradit Chinese Me, Lanzhou 730000, Peoples R China;[4]corresponding author), MOE PRC, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2025

卷号：15

期号：9

外文期刊名：METABOLITES

收录：;Scopus(收录号：2-s2.0-105017381284);WOS:【SCI-EXPANDED(收录号:WOS:001580358400001)】；

基金：This study was supported by Gansu Provincial Science and Technology Major Project (23ZDFA013-1), Strategic Research and Consulting Project of the Chinese Academy of Engineering (GS2021ZDA06-2), Gansu Provincial Department of Education 'Double First-Class' Key Scientific Research Project (GSSYLXM-05), Gansu Provincial University Youth Doctoral Fund funded project (2023QB-094), Gansu Provincial Science and Technology Project (20JR5RA182), and Gansu Province University Teacher Innovation Fund Project (2025A-116).

语种：英文

外文关键词：Angelica sinensis; wild; cultivated; phenylpropanoid biosynthesis; flavonoid biosynthesis

摘要：Objectives: Angelica sinensis is a type of traditional Chinese medicine (TCM) used primarily as a blood tonic. The chemical components that exert their efficacy are mainly bioactive metabolites, such as ferulic acid, flavonoids, and volatile oils. The resources of wild Angelica sinensis (WA) are very scarce, and almost all the market circulation of TCM formulations relies on cultivated Angelica sinensis (CA). Some studies have shown that WA and CA differ in morphological features and chemical composition, but the reasons and mechanisms behind the differences have not been studied deeply. Methods: Herein, metabolomics analysis (MA) and transcriptomics analysis (TA) were used to reveal the differences in bioactive metabolites and genes between WA and CA. Expression of key genes was verified by real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Results: Results showed that 12,580 differential metabolites (DMs) and 1837 differentially expressed genes (DEGs) were identified between WA and CA. Fourteen DMs (e.g., cinnamic acid, caffeic acid, ferulic acid, p-coumaroylquinic acid, and phlorizin) and 27 DEGs (e.g., cinnamic acid 4-hydroxylase (C4H), 4-coumarate-CoA ligase (4CL), shikimate O-hydroxycinnamoyltransferase (HCT), caffeic acid-O-methyltransferase (COMT), cinnamyl-alcohol dehydrogenase (CAD), flavonol synthase (FLS)) were screened in phenylpropanoid biosynthesis and flavonoid biosynthesis. A combined analysis of MA and TA was performed, and a network map of DMs regulated by DEGs was plotted. The results of real-time RT-qPCR showed that the transcriptome data were reliable. Conclusions: These findings provide a reference for further optimization of the development of WA cultivation and breeding of CA varieties.