文献类型：期刊文献

中文题名：基于混合测序策略的两品种当归中类黄酮调控基因差异分析

英文题名：Differential Analysis of Flavonoid-regulated Genes in Two Cultivars of Angelica sinensis Based on Hybrid Sequencing Strategy

作者：徐丽[1];朱田田[1,2,3];晋玲[1,2,3];康舒淇[1];张明惠[1];王圆圆[1]

第一作者：徐丽

机构：[1]甘肃中医药大学,兰州730000;[2]西北中藏药省部共建协同创新中心,兰州1730000;[3]甘肃省珍稀中药资源评价与保护利用工程研究中心,兰州730000

第一机构：甘肃中医药大学

年份：2023

卷号：29

期号：1

起止页码：141

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2020】；

基金：甘肃省科技计划项目(20JR5RA182);道地药材生态种植及质量保障项目(国中医药科技[2020]153号);甘肃省教育科技创新项目(GSSYLXM-05);甘肃中医药大学科学研究与创新基金项目(2021KCZD-4);西北中藏药省部共建协同创新中心开放基金(Xbzzy202207)。

语种：中文

中文关键词：当归;全长转录组;RNA测序技术;类黄酮生物合成;差异表达基因

外文关键词：Angelica sinensis;full-length transcriptome;RNA sequencing technology;flavonoid biosynthesis;differentially expressed genes

摘要：目的:比较2个不同茎叶色当归品种岷归1号与岷归2号转录水平差异。方法:以2种颜色当归的新鲜叶片(带叶柄)和上端茎为材料,采用混合测序策略,应用全长转录组技术构建当归无参全长转录本文库,利用RNA-seq技术对两品种进行差异基因表达分析,再利用公共数据库对差异基因的生物学功能进行注释和精细分类,筛选调控当归茎叶色差异的主要候选基因。结果:当归转录本的测序结果良好,测序数据质量较高,当归全长转录本的34528条序列在非冗余蛋白(NR)、京都基因与基因组百科全书(KEGG)、SwissProt及KOG数据库中分别注释到33947、33241、29150和22601条。对两品种当归差异表达基因(DEGs)进行精细分类,具有生物学功能和分子功能的705个DGEs可分为11类,主要富集在初级代谢(17.87%)、逆境响应(14.47%)、次级代谢(11.49%)等功能上,与颜色有关的差异表达基因主要集中在类黄酮生物合成途径上。结论:两品种当归茎叶颜色差异的主要原因可能与调控类黄酮的生物合成基因的表达差异有关,可为后续进行功能验证,进一步明确与当归主要药效成分之间的联系奠定基础。
Objective:To compare the transcriptional levels of two cultivars(Mingui 1 and Mingui 2with different stem and leaf colors.Method:The fresh leaves with petioles and the upper stems from Angelica sinensis with two colors were selected as the material,and the hybrid sequencing strategy was used.The technology at the transcriptome was used to build the non-reference full-length transcript library of A.sinensis,and the RNA-seq technology was used to analyze differentially expressed genes of the two cultivars,reusing a public database for biology function annotation and fine classification of differentially expressed genes.The main candidate genes regulating color differences between stems and leaves of A.sinensis were screened out.Result:The sequencing results of A.sinensis transcripts were good and the quality of the sequencing data was high.The34528 full-length transcripts were annotated into 33947,33241,29150,and 22601 in the Non-redundant Protein Sequence Database(NR),Kyoto Encyclopedia of Genes and Genomes(KEGG),SwissProt,and Clusters of Orthologous Groups for Eukaryotic Complete Genomes(KOG),respectively.The 705 differentially expressed genes of the two cultivars with biological and molecular functions were divided into 11 categories,which were mainly enriched in the primary metabolism(17.87%),stress response(14.47%),and secondary metabolism(11.49%).The differentially expressed genes related to colors were mainly concentrated in the flavonoid biosynthesis pathway.Conclusion:The main reason for the color differences of the stems and leaves in two cultivars of A.sinensis may be related to the expression differences of genes regulating flavonoid biosynthesis,which lays a foundation for subsequent functional verification and further clarification of the relationship with the main pharmacodynamic components of A.sinensis.