文献类型：期刊文献

中文题名：敦煌医方大补脾汤对放射性肠水肿大鼠的防护作用研究

英文题名：Study on the Protective Effect of the Dunhuang Medical Formula Dabupi Decoction on Rats with Radiation-induced Intestinal Edema

作者：张利英[1,2];李洋洋[1];王欣[1];苗致铭[1];张尚祖[1];陈琰[1];杨更强[1];李启杨[1];刘富仙[1];刘永琦[1,2]

第一作者：张利英

机构：[1]甘肃中医药大学,甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,兰州730000;[2]甘肃中医药大学,敦煌医学与转化教育部重点实验室,兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2025

卷号：42

期号：1

起止页码：13

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金联合项目(U23A20502);敦煌医学与转化教育部重点实验室开放课题(DHYX24-23、DHYX23-09);甘肃省“创新之星”项目(2022CXZX-735)。

语种：中文

中文关键词：敦煌医方;大补脾汤;放射性肠水肿;防护作用

外文关键词：Dunhuang medical formula;Dabupi decoction;radiation-induced intestinal edema;protective function

摘要：目的探明敦煌医方大补脾汤对放射性肠水肿的防护作用及可能机制,为临床放射性肠水肿的防护及敦煌医方的推广提供实验依据。方法将SD大鼠分成3组,分别为空白对照组、模型组和实验组,模型组采用6 Gy X射线进行全身照射,实验组在照射前14 d给予大补脾汤6.8 g·kg-1灌胃。观察大鼠一般状况;HE染色观察结肠组织形态变化;透射电镜检测结肠组织超微结构变化;免疫荧光检测结肠组织中ROS、缺氧诱导因子1α(hypoxia inducible factor 1 alpha,HIF1α)和水通道蛋白(aquaporin,AQP4)表达水平;蛋白免疫印迹法检测结肠组织HIF-1α、AQP4、泛素化连接酶(heme oxidized IRP2 ubiquitin ligase 1 like,HOIL-1L)、蛋白激酶Cζ(protein kinase C zeta,PKCζ)、Occludin和钠-钾ATP酶(Na+/K+ATPase)蛋白的表达水平。结果与空白对照组相比,模型组大鼠结肠组织出现了黏膜坏死脱落、黏膜及黏膜下水肿,结肠超微结构显示细胞质基质肿胀,线粒体肿胀变形,扩张形成空泡化,ROS、HIF-1α和AQP4荧光表达显著升高(P<0.05或P<0.01),HIF-1α、AQP4和HOIL-1L蛋白表达显著升高(P<0.01)以及PKCζ、Occludin和Na+/K+-ATPase蛋白表达显著降低(P<0.01)。与模型组相比,实验组结肠组织黏膜脱落和水肿现象减轻,结肠线粒体肿胀明显好转,胞质内大面积空泡现象明显改善,ROS、HIF-1α和AQP4荧光表达显著降低(P<0.05或P<0.01),HIF-1α、AQP4和HOIL-1L蛋白表达显著降低(P<0.01)以及PKCζ、Occludin和Na+/K+-ATPase蛋白表达显著升高(P<0.01)。结论敦煌医方大补脾汤可以有效防护放射性肠水肿,其机制可能是通过抑制ROS、HIF-1α和AQP4的表达,促进PKCζ、Na+/K+-ATPase以及Occludin的表达来发挥作用。
OBJECTIVE To investigate the protective effect and possible mechanism of Dunhuang medical formula Dabupi decoction on radiation-induced intestinal edema,and to provide experimental basis for the protection of clinical radiation-induced intestinal edema and the promotion of Dunhuang medical formula.METHODS SD rats were divided into three groups,blank control group,model group and experimental group,the model group was irradiated whole body by 6 Gy X-ray,and the experimental group was given Dabupi decoction 6.8 g·kg?1 by gavage 14 d before irradiation.The general conditions of rats were observed;morphological changes of colon tissues were observed by HE staining;ultrastructural changes of colon tissues were detected by transmission electron microscopy;the expression levels of ROS,hypoxia inducible factor 1 alpha(HIF-1α)and aquaporin(AQP4)in colon tissues were detected by immunofluorescence;and the expression levels of HIF-1α,AQP4,heme oxidized IRP2 ubiquitin ligase 1 like(HOIL-1L),protein kinase C zeta(PKCζ),Occludin and Na+/K+-ATPase protein were detected by Western blotting.RESULTS Compared with the blank control group,the colon tissues of rats in the model group showed mucosal necrosis and detachment,mucosal and submucosal oedema,and the ultrastructure of the colon showed swollen cytoplasmic matrix,swollen and deformed mitochondria,dilated to form vacuolisation,significantly higher fluorescence expression of ROS,HIF-1αand AQP4(P<0.05 or P<0.01),HIF-1α,AQP4 and HOIL-1L protein expression was significantly higher(P<0.01),while PKCζ,Occludin and Na+/K+-ATPase protein expression was significantly lower(P<0.01).Compared with the model group,the experimental group showed reduced mucosal detachment and oedema in colonic tissues,significantly better swelling of colonic mitochondria,significantly improved large intracytoplasmic vacuoles,significantly lower fluorescence expression of ROS,HIF-1α,and AQP4(P<0.05 or P<0.01),and significantly decreased protein expression of HIF-1α,AQP4,and HOIL-1L(P<0.01),while significantly increased protein expression of PKCζ,Occludin and Na+/K+-ATPase(P<0.01).CONCLUSION The Dunhuang medical formula Dabupi decoction can effectively protect radiation-induced intestinal edema,and the mechanism may work by inhibiting the expression of ROS,HIF-1αand AQP4 and promoting the expression of PKCζ,Na+/K+-ATPase and Occludin.