文献类型：期刊文献

英文题名：Tubuloside B, a major constituent of Cistanche deserticola , inhibits migration of hepatocellular carcinoma by inhibiting Hippo-YAP pathway

作者：Yao, Jie[1,2,3,4];Wan, Haoqiang[1,2,3,4];Zhang, Jingmei[1,2,3,5];Shen, Wanying[1,2,3,6];Wei, Xiaofang[1,2,3,6];Shi, Chenyan[1,2,3,4];Ou, Baoru[1,2,3,4];Liu, Dongyu[1,2,3,4];Ge, Lanlan[1,2,3,4];Fei, Jia[7];Zeng, Xiaobin[1,2,3,4]

第一作者：Yao, Jie

通信作者：Zeng, XB[1];Zeng, XB[2];Zeng, XB[3];Zeng, XB[4];Fei, J[5]

机构：[1]Jinan Univ, Shenzhen Clin Res Ctr Geriatr, Dept Hepatobiliary & Pancreat Surg, Shenzhen 518020, Guangdong, Peoples R China;[2]Jinan Univ, Shenzhen Peoples Hosp, Longhua Branch, Clin Med Coll 2,Ctr Lab, Shenzhen 518020, Guangdong, Peoples R China;[3]Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China;[4]Jinan Univ, Shenzhen Peoples Hosp, Dept Pathol, Clin Med Coll 2, Shenzhen 518120, Guangdong, Peoples R China;[5]Guangzhou Univ Chinese Med, Shenzhen Hosp Integrated Tradit Chinese & Western, Shenzhen 518104, Guangdong, Peoples R China;[6]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 73000, Gansu, Peoples R China;[7]Jinan Univ, Med Coll, Dept Biochem & Mol Biol, Guangzhou 510632, Peoples R China

第一机构：Jinan Univ, Shenzhen Clin Res Ctr Geriatr, Dept Hepatobiliary & Pancreat Surg, Shenzhen 518020, Guangdong, Peoples R China

通信机构：[1]corresponding author), Jinan Univ, Shenzhen Clin Res Ctr Geriatr, Dept Hepatobiliary & Pancreat Surg, Shenzhen 518020, Guangdong, Peoples R China;[2]corresponding author), Jinan Univ, Shenzhen Peoples Hosp, Longhua Branch, Clin Med Coll 2,Ctr Lab, Shenzhen 518020, Guangdong, Peoples R China;[3]corresponding author), Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China;[4]corresponding author), Jinan Univ, Shenzhen Peoples Hosp, Dept Pathol, Clin Med Coll 2, Shenzhen 518120, Guangdong, Peoples R China;[5]corresponding author), Jinan Univ, Med Coll, Dept Biochem & Mol Biol, Guangzhou 510632, Peoples R China.

年份：2024

卷号：129

外文期刊名：PHYTOMEDICINE

收录：;Scopus(收录号：2-s2.0-85188953402);WOS:【SCI-EXPANDED(收录号:WOS:001224467500001)】；

基金：This work was supported by National Natural Science Foundation of China (81503221, 81903760, 82104498, and 82204682), Natural Science Foundation of Guangdong Province (2017A030313659, 2021A1515220185, and 2021A1515110841), Shenzhen Fundamental Research and Discipline Layout Project (JCYJ20210324113003007, JCYJ20220530152011025, GJHZ20220913142618035, and JCYJ20220818102609021).r Research and Discipline Layout Project (JCYJ20210324113003007, JCYJ20220530152011025, GJHZ20220913142618035, and JCYJ20220818102609021) .

语种：英文

外文关键词：Tubuloside B; Hepatocellular carcinoma; Migration; Hippo pathway; YAP

摘要：Background: Studies have shown that phenylethanoid glycosides (PhGs) have multiple pharmacological effects such as anti-inflammatory, hepatoprotective or neuroprotective functions, whereas their anti -tumor effects are rarely studied. Tubuloside B (Tub B) is a PhG isolated from Cistanche deserticola , a traditional Chinese medicine. To date, there is a lack of comprehensive research regarding the biological activity of Tub B. Purpose: The subject of the current study was to investigate the anti-hepatocellular carcinoma (HCC) cell activity and the underlying mechanism of Tub B. Methods: We evaluated the in vitro anti-migratory effect of Tub B by scratch and transwell assays. RNA-seq was employed to identify the differential genes by Tub B. Besides, the functional mechanism of Tub B was investigated by distinct molecular biology techniques including immunofluorescent staining, quantitative PCR, as well as western blot analysis. Subsequently, we utilized Hep3B cells for in vivo metastasis assays through spleen injection and evaluated the anti-migratory effect of Tub B in hepatocellular carcinoma (HCC). Results: Tub B exhibited in vitro and in vivo inhibition of HCC cell migration. Tub B decreased the expression of transcriptional target genes downstream of the Hippo pathway, including CTGF, CYR61, and N-cadherin as determined by RNA-seq. Furthermore, mechanistic studies confirmed that Tub B increased phosphorylation of YAP at S127, which contributes to YAP cytoplasmic localization. Additionally, overexpression of YAP abrogated Tub B-induced inhibition of HCC migration and the mRNA levels of CTGF, CYR61, and N-cadherin . Conclusions: Taken together, these results illustrated that Tub B demonstrated great potential in inhibiting migration of HCC, and a portion of its impact can be attributed to the modulation of the Hippo-YAP pathway.