文献类型：期刊文献

中文题名：肝郁脾虚证模型大鼠免疫细胞分化和增殖变化规律研究

英文题名：Rule of Proliferation and Differentiation of Immune Cells in Rats Model of Liver Depression and Spleen Deficiency

作者：刘建鸿[1];岳嘉[1];段永强[1];夏琦[1];程小丽[1]

第一作者：刘建鸿

机构：[1]甘肃中医学院

第一机构：甘肃中医药大学

年份：2013

卷号：20

期号：1

起止页码：43

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2013_2014】；

基金：甘肃省科技厅自然科学基金(0803RJZA071)

语种：中文

中文关键词：肝郁脾虚证;外周血细胞;骨髓象;骨髓细胞周期;大鼠

外文关键词：syndrome of liver depression and spleen deficiency; peripheral blood cell; myelogram; marrow cell circle; rats

摘要：目的探讨实验条件下的肝郁脾虚证大鼠骨髓和外周血淋巴细胞和中性粒细胞分化和增殖的变化规律。方法采用夹尾激怒、束缚应激、过度疲劳与饮食失节的复合方法复制肝郁脾虚证动物模型。检测空白对照组和模型2、6、9周组雄性大鼠外周血象、骨髓象和骨髓细胞增殖周期。结果外周血象显示:与空白对照组比较,模型大鼠2周组白细胞和中性粒细胞数量升高(P<0.05,P<0.01),9周组淋巴细胞数量降低(P<0.05)。骨髓象显示:与空白对照组比较,模型大鼠2周组成熟中性粒细胞升高、淋巴细胞比例降低(P<0.05),9周组淋巴细胞比例升高(P<0.05)。骨髓细胞周期显示:与空白对照组比较,模型大鼠2周组S期和G2/M期升高(P<0.05),2周、6周组增殖指数升高(P<0.05,P<0.01)。结论肝郁脾虚因素会影响机体免疫细胞的分化和增殖,早期可促进骨髓增殖。
Objective To explore the rule proliferation and differentiation of lymphocyte and neutrophil in peripheral blood cell and myelogram of rats with syndrome of liver depression and spleen deficiency.Methods Rat model with syndrome of liver depression and spleen deficiency was made by composite method of angered by clipping tails,restraint stress,over fatigue and abnormal diet.The hemogram,myelogram and marrow cell circle of normal group and model group were measured at 2nd,6th and 9th week.Results Hemogram showed：leukocyte and neutrophils in model group of 2 weeks was significantly higher than that in normal group（P〈0.05 or P〈0.01）,lymphocyte in model group of 9 weeks was significantly lower than that in normal group（P〈0.05）.The myelogram showed：neutrophils in model group of 2 weeks was significantly higher than that in normal group（P〈0.05）,lymphocyte in model group of 2 weeks was significantly lower than that in normal group（P〈0.05）,lymphocyte in model group of 9 weeks was significantly higher than that in normal group（P〈0.05）.The detection of marrow cell circle showed：The S phase and G2/M phase in model group of 2 weeks was significantly higher than that in normal group（P〈0.05）,the proliferation index in model group of 2 weeks and 6 weeks were significantly higher than that in normal group（P〈0.05）.Conclusion The factor of liver depression and spleen deficiency can affect proliferation and differentiation of immune cells.It can promote proliferation of bone marrow in early stage.