文献类型：期刊文献

英文题名：Comparative study on the gastrointestinal- and immune- regulation functions of Hedysari Radix Paeparata Cum Melle and Astragali Radix Praeparata cum Melle in rats with spleen-qi deficiency, based on fuzzy matter-element analysis

作者：Zhang, Yugui[1,2];Niu, Jiangtao[1,2];Zhang, Shujuan[1,2];Si, Xinlei[1,2];Bian, Tian-Tian[1,2];Wu, Hongwei[1,2];Li, Donghui[1,2];Sun, Yujing[1,2];Jia, Jing[3];Xin, Erdan[1,2];Yan, Xingke[3];Li, Yuefeng[1,2,4]

第一作者：张永刚

通信作者：Li, YF[1];Li, YF[2];Yan, XK[3];Li, YF[4]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[2]Gansu Univ Chinese Med, Key Lab Stand & Qual Chinese Med Res Gansu, Engn Res Ctr Chinese Med Pharmaceut Proc Gansu, Lanzhou, Peoples R China;[3]Gansu Univ Chinese Med, Mol Biol Lab, Coll Acupuncture Moxibust & Tuina, Lanzhou, Peoples R China;[4]Gansu Univ Chinese Med, Sci Res & Expt Ctr, Lanzhou, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Key Lab Stand & Qual Chinese Med Res Gansu, Engn Res Ctr Chinese Med Pharmaceut Proc Gansu, Lanzhou, Peoples R China;[3]corresponding author), Gansu Univ Chinese Med, Mol Biol Lab, Coll Acupuncture Moxibust & Tuina, Lanzhou, Peoples R China;[4]corresponding author), Gansu Univ Chinese Med, Sci Res & Expt Ctr, Lanzhou, Peoples R China.|[107359c91a78c5fd2e803]甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）;[10735]甘肃中医药大学;[10735db5df93766a96e5b]甘肃中医药大学针灸推拿学院;[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;

年份：2022

卷号：60

期号：1

起止页码：1237

外文期刊名：PHARMACEUTICAL BIOLOGY

收录：;Scopus(收录号：2-s2.0-85133143956);WOS:【SCI-EXPANDED(收录号:WOS:000817943300001)】；

基金：This study was supported by the National Natural Science Foundation of China [81960713, 82160750], Gansu Provincial Education Department Industrial Support Plan Project [2021CYZC21], the Chinese Medicine Pharmaceutical Process Engineering Research Center Open Fund of Gansu [ZYGY202003], Gansu Provincial Department of Science and Technology-Science and Technology Plan (Innovation Base and Talent Plan) Basic Research Innovation Group Project [21JR7RA569].

语种：英文

外文关键词：Traditional Chinese medicinal herb; tonifying middle and replenishing qi; the efficacy difference; gastrointestinal dysfunction; immune disorders; mathematical language

摘要：Context Hedysari Radix Praeparata Cum Melle (HRPCM) and Astragali Radix Praeparata Cum Melle (ARPCM) are used interchangeably in clinics to treat spleen-qi deficiency (SQD) symptom mainly including gastrointestinal dysfunction and decreased immunity, which has unknown differences in efficacy. Objective To investigate the differences between HRPCM and ARPCM on intervening gastrointestinal- and immune-function with SQD syndrome. Materials and methods After the SQD model was established, the Sprague-Dawley (SD) rats were randomly divided into nine groups (n = 10): normal; model; Bu-Zhong-Yi-Qi Pills; 18.9, 12.6 and 6.3 g/kg dose groups of HRPCM and ARPCM. Gastrointestinal function including d-xylose, gastrin, amylase vasoactive intestinal peptide, motilin, pepsin, H+/K+-ATPase, Na+/K+-ATPase, sodium-glucose cotransporter 1 (SGLT1), glucose transporter 2 (GLUT2) and immune function including spleen and thymus index, blood routine, interleukin (IL)-2, IL-6, interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha), immunoglobulin (Ig) M, IgA, IgG and delayed-type hypersensitivity (DTH) were detected. Finally, the efficacy differences were analysed comprehensively by the fuzzy matter-element method. Results In regulating immune, the doses differences in efficacy between HRPCM and ARPCM showed in the high-dose (18.9 g/kg), but there were no differences in the middle- and low- dose (12.6 and 6.37 g/kg); the efficacy differences were primarily reflected in levels of IL-6, IFN-gamma, TNF-alpha and IgM in serum, and the mRNA expression of IL-6 and IFN-gamma in the spleen. In regulating gastrointestinal, the efficacy differences were primarily reflected in the levels of D-xylose, MTL, and GAS in serum, and the mRNA and protein expression of SGLT1 and GLUT2 in jejunum and ileum. Discussion and conclusions HRPCM is more effective than ARPCM on regulating gastrointestinal function and immune function with SQD syndrome. Therefore, we propose that HRPCM should be mainly used to treat SQD syndrome in the future.