文献类型：期刊文献

中文题名：败酱草多糖的脱色工艺和抗氧化活性研究

英文题名：Study on decolorization process for polysaccharide from Herba Patriniae and antioxidation activity

作者：惠和平[1,2];李晓东[3];史彦斌[4];张艳[5];马江鹏[1];曹泽钰[1];唐东科[1];常红霞[1];王娟弟[1]

第一作者：惠和平

机构：[1]甘肃农业职业技术学院,甘肃兰州730020;[2]中国科学院西北特色植物资源化学重点实验室,甘肃省天然药物重点实验室,兰州化学物理研究所,甘肃兰州730000;[3]甘肃省中医院中医药研究院中药研究所,甘肃兰州730050;[4]兰州大学,甘肃兰州730000;[5]甘肃中医药大学,甘肃兰州730010

第一机构：甘肃农业职业技术学院,甘肃兰州730020

年份：2021

卷号：41

期号：S01

起止页码：231

中文期刊名：现代化工

外文期刊名：Modern Chemical Industry

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD_E2021_2022】；

基金：甘肃省大学生创新训练项目(2020DC-GNZY-20);甘肃省高等学校基本科研业务费专项(甘财教2014-63);陇原青年创新创业人才项目(2108-81);甘肃农业职业技术学院科研流动站(2017-GNZY-04)

语种：中文

中文关键词：败酱草;多糖;脱色工艺;抗氧化

外文关键词：Herba Patriniae;polysaccharide;decolorization process;antioxidation

摘要：以败酱草粗多糖为原料,在单因素实验的基础上,采用正交实验比较H_(2)O_(2)和活性炭对败酱草多糖的脱色效果,优选最佳脱色工艺。以Vc为对照,运用体外抗氧化评价体系评价败酱草多糖脱色前后的抗氧化活性。结果表明,活性炭的脱色效果较好,其最佳工艺为:活性炭用量5%,脱色温度65℃,脱色时间120 min;在此条件下,多糖的脱色率和得率分别为50.21%±1.32%和70.36%±1.25%。所得败酱草多糖对DPPH·和·OH均有一定的清除作用,且脱色后败酱草多糖的清除能力均强于未脱色多糖;尤其在低浓度时(<0.5 mg/m L),脱色后败酱草多糖清除DPPH·和·OH的能力强于Vc,且清除能力与多糖的浓度呈正相关。
Based on single factor experiment,orthogonal test method is utilized to compare the decolorization effects of H_(2)O_(2) and activated carbon on crude polysaccharide from Herba Patriniae as and raw material,and the best decolorizer and decolorization process is preferred.Taking Vc as a control,DPPH·and hydroxyl radical(·OH)scavenging experiments are performed to evaluate the antioxidation activity of Herba Patriniae polysaccharides before and after decolorization.Results verify that activated carbon presents better decolorization effect.The optimum process is obtained as follows:the dosage of activated carbon is 5%,decolorization temperature remains at 65℃,and the decolorization lasts for120 min.Under these conditions,the decolorization rate and the yield of polysaccharide are 50.21%±1.32%and70.36%±1.25%,respectively.The obtained polysaccharide presents a certain scavenging effect on DPPH·and·OH radicals,and decolored polysaccharide shows a stronger scavenging ability on two kinds of free radicals than undecolored polysaccharide.At low concentrations(<0.5 mg·m L^(-1)),the ability of decolored polysaccharide to scavenge DPPH and·OH radicals is stronger than that of Vc,and there is a positive dose-depending relationship between the concentration of polysaccharide and its scavenging ability to DPPH·and·OH radicals.