文献类型：期刊文献

中文题名：Meta-Analysis of Polymerase Chain Reaction in the Detection of Human Leptospirosis

作者：MA Lina[1,2];MA Ya[3];LUO Bailing[2];MA Bishu[1,4];YAN Jie[1];LIN Xu'ai[1]

第一作者：MA Lina;马丽娜;麻丽娜

机构：[1]School of Medicine,Zhejiang University,Hangzhou 310058,Zhejiang,China;[2]Department of Medical Teaching in Dingxi Campus,Gansu University of Chinese Medicine,Dingxi 743000,Gansu,China;[3]Department of Petroleum Chemical Engineering,Lanzhou Petrochemical College of Vocational Technology,Lanzhou 730060,Gansu,China;[4]Department of Medical Microbiology and Immunology,Haiyuan School of Kunming Medical University,Kunming 650106,Yunnan,China

第一机构：School of Medicine,Zhejiang University,Hangzhou 310058,Zhejiang,China

年份：2020

卷号：25

期号：5

起止页码：387

中文期刊名：武汉大学自然科学学报：英文版

收录：CSTPCD;;Scopus;CSCD:【CSCD2019_2020】；

基金：the National Natural Science Foundation of China(81271781);the Natural Science Foundation of Zhejiang Province(LY17H190002);the Higher Education Institutions of Gansu Province of China(2019B-230)。

语种：英文

中文关键词：human leptospirosis;polymerase chain reaction;meta-analysis;sensitivity;specificity

摘要：Leptospirosis,a major zoonotic disease widespread in the world,would infect humans and other animals by direct contacting with the soil or water contaminated by the pathogenic leptospires.This report analyzes the accuracy of polymerase chain reaction(PCR)assay used in the detection of leptospira specific genes in humans.Seventeen published articles which included 2526 samples satisfied all inclusion standards and were applied to meta-analysis.Pooled sensitivity and specificity were 0.73(95%CI 0.70-0.76)and 0.94(95%CI 0.93-0.95),respectively.Heterogeneity was statistically significant among studies,but none of the sources for heterogeneity(disease stage,PCR type,targeted genes)could adequately interpret this finding.Meta-regression advised that real-time PCR(qPCR)targeted 16 s rRNA would be the best choice for early detection of cases during the acute stage of the disease and was accounted as a good screening tool in all stages of the disease.