文献类型：期刊文献

中文题名：敦煌泻心汤对溃疡性结肠炎小鼠的治疗作用

英文题名：Therapeutic effects of Dunhuang Xiexin decoction on mice with ulcerative colitis

作者：杜芹[1];高榕妮[2];李爽[1];李亮亮[1];蔺兴遥[2,3]

第一作者：杜籼芹

机构：[1]甘肃中医药大学基础医学院,甘肃兰州730000;[2]甘肃中医药大学中医临床学院,甘肃兰州730000;[3]甘肃中医药大学敦煌医学与转化教育部重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2025

卷号：41

期号：3

起止页码：376

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：;北大核心:【北大核心2023】；

基金：甘肃省人才发展专项基金资助项目(2021RCXM106)。

语种：中文

中文关键词：敦煌泻心汤;敦煌医学;溃疡性结肠炎;葡聚糖硫酸钠;肠道屏障;核因子-κB

外文关键词：Dunhuang Xiexin decoction;Dunhuang medicine;ulcerative colitis;dextran sulfate sodium;intestinal barrier;nuclear factor kappa-B

摘要：目的探究敦煌泻心汤对溃疡性结肠炎(UC)小鼠的治疗作用及其机制。方法将小鼠随机分为空白组(0.9%NaCl)、模型组(0.9%NaCl)和低、中、高剂量实验组(2.93、5.85、11.70 g·kg^(-1)敦煌泻心汤水提物),每组10只,连续给药7 d。同时,除空白组外,其余4组小鼠自由饮用含3%葡聚糖硫酸钠的饮用水,连续7 d,构建UC模型。用酶联免疫吸附试验法测定小鼠血清中肿瘤坏死因子α(TNF-α)、白细胞介素-1β(IL-1β)和IL-6水平,用蛋白质印迹法检测结肠组织中闭合蛋白(occludin)、闭锁小带蛋白1(ZO-1)与磷酸化核因子-κB p65(p-NF-κB p65)蛋白的表达水平。结果中、高剂量实验组和模型组、空白组血清中TNF-α水平分别为(397.57±33.05)、(389.41±22.57)、(472.19±18.70)和(355.92±31.86)pg·mL^(-1),IL-1β水平分别为(73.06±18.38)、(62.60±9.80)、(98.94±27.50)和(55.26±18.14)ng·mL^(-1),IL-6水平分别为(153.23±32.96)、(148.12±23.53)、(239.35±50.99)和(131.36±27.58)pg·mL^(-1),结肠组织中occludin蛋白相对表达水平分别为0.80±0.13、1.03±0.24、0.57±0.15和1.10±0.03,ZO-1蛋白相对表达水平分别为0.90±0.21、1.09±0.10、0.73±0.16和1.29±0.03,p-NF-κB p65蛋白相对表达水平分别为0.75±0.12、0.53±0.08、1.15±0.19和0.43±0.21。高剂量实验组的上述指标与模型组比较,在统计学上差异均有统计学意义(均P<0.05)。结论敦煌泻心汤可能通过修复肠道屏障及抑制NF-κB信号通路减少炎症因子释放来改善小鼠UC。
Objective To explore the therapeutic effects and mechanisms of Dunhuang Xiexin decoction on mice with ulcerative colitis(UC).Methods Mice were randomly divided into blank group(0.9%NaCl),model group(0.9%NaCl)and experimental-L,-M,-H groups(2.93,5.85,11.70 g·kg^(-1)Dunhuang Xiexin decoction water extracts)with 10 cases per group for 7 days.Meanwhile,apart from the blank group,the other four groups of mice were allowed adlibitum access to drinking water containing 3%sodium dextran sulfate for 7 consecutive days to establish the UC model.The levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and IL-6 in mouse serum were determined by enzyme-linked immunosorbent assay.The expression levels of occludin,zonula occludens-1(ZO-1)and phospho nuclear factor kappa B p65(p-NF-κB p65)in colonic tissues was detected by Western blot.Results The serum TNF-αlevels in the experimental-M,-H groups,model group and blank group were(397.57±33.05),(389.41±22.57),(472.19±18.70)and(355.92±31.86)pg·mL^(-1);the IL-1βlevels were(73.06±18.38),(62.60±9.80),(98.94±27.50)and(55.26±18.14)ng·mL^(-1);the IL-6 levels were(153.23±32.96),(148.12±23.53),(239.35±50.99)and(131.36±27.58)pg·mL^(-1);the relative expressions levels of occludin protein in colon tissues were 0.80±0.13,1.03±0.24,0.57±0.15 and 1.10±0.03;the relative expressions levels of ZO-1 protein were 0.90±0.21,1.09±0.10,0.73±0.16 and 1.29±0.03;the relative expressions levels of p-NF-κB p65 protein were 0.75±0.12,0.53±0.08,1.15±0.19 and 0.43±0.21,respectively.There were significant differences between the experimental-H group and the model group(P<0.05).Conclusion Dunhuang Xiexin decoction may improve ulcerative colitis in mice by repairing the intestinal barrier and inhibiting the NF-κB signaling pathway to reduce the release of inflammatory factors.