文献类型：期刊文献

英文题名：ASYMMETRIC LEAVES2 gene, a member of LOB/AS2 family of Arabidopsis thaliana, causes an abaxializing leaves in transgenic cockscomb

作者：Sun, Shao-Bo[2];Song, Jiang-Ping[3];Meng, Lai-Sheng[1,2]

第一作者：孙少伯

通信作者：Meng, LS[1]

机构：[1]Pohang Univ Sci & Technol, Div Mol & Life Sci, Pohang 790784, Kyungbuk, South Korea;[2]Gansu Coll Tradit Chinese Med, Lanzhou 730000, Peoples R China;[3]Shaoxing Univ, Sch Yuanpei, Shaoxing 312000, Peoples R China

第一机构：Pohang Univ Sci & Technol, Div Mol & Life Sci, Pohang 790784, Kyungbuk, South Korea

通信机构：[1]corresponding author), Pohang Univ Sci & Technol, Div Mol & Life Sci, Pohang 790784, Kyungbuk, South Korea.

年份：2012

卷号：39

期号：4

起止页码：4927

外文期刊名：MOLECULAR BIOLOGY REPORTS

收录：;Scopus(收录号：2-s2.0-84863084120);WOS:【SCI-EXPANDED(收录号:WOS:000301108500178)】；

语种：英文

外文关键词：ASYMMETRIC LEAVES2 (AS2); Arabidopsis thaliana; Cockscomb (Celosia cristata) ab-adaxial polarity; Malformed leaves; Overexpression

摘要：The leaf primordium derives from the peripheral zone of shoot apical meristem. During the formation of leaf primordia, they need to establish the proximodistal, mediolateral, and ab/adaxial axes. Among these axes, the ab/adaxial axis might be the most important. ASYMMETRIC LEAVES2 (AS2) gene is a member of AS2/LATERAL ORGAN BOUNDARY (LOB) family of Arabidopsis thaliana. In this work, we transformed 35S:AS2 transgene constructs to cockscomb (Celosia cristata) via Agrobacterium tumefaciens. All primary transformants subsequently obtained were placed into phenotypic categories and self-pollinated. As a whole, a total of 44 T1 35S:AS2 cockscomb plants obtained were grouped into two major categories: (I) slightly wrinkled leaves (28/44), (II) extremely curved leaves (16/44), on the basis of their leaf phenotypes. Furthermore, we characterized the anatomical features of these malformed leaves; and found the transformation of adaxial cell types into abaxial cell ones. A series of data suggest that AS2 might be involved in the determination of abaxial polarity in cockscomb plants. However, a few research teams have reported that AS2 might be involved in the determination of adaxial polarity in leaf primodia of Arabidopsis thaliana. These data above indicate that the roles of the same ab/adaxial determinant might differ between distinct species. At last, the different function of AS2 in distinct species was discussed.