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地龙蛋白调节RhoA/Rho信号通路改善DMED大鼠勃起功能的机制研究     被引量:2

Earthworm protein improves erectile function of DMED rats via regulating RhoA/Rho signaling pathway

文献类型:期刊文献

中文题名:地龙蛋白调节RhoA/Rho信号通路改善DMED大鼠勃起功能的机制研究

英文题名:Earthworm protein improves erectile function of DMED rats via regulating RhoA/Rho signaling pathway

作者:剡锐[1,2];张爱平[2];刘黎明[2];冀小卫[2];陈小勇[1];郭庆华[1];王新平[3];邢喜平[3]

第一作者:剡锐

机构:[1]兰州大学第一医院,甘肃兰州730000;[2]甘肃中医药大学,甘肃兰州730000;[3]甘肃中医药大学附属医院,甘肃兰州730020

第一机构:兰州大学第一医院,甘肃兰州730000

年份:2023

卷号:37

期号:5

起止页码:32

中文期刊名:中国男科学杂志

外文期刊名:Chinese Journal of Andrology

收录:CSTPCD;;CSCD:【CSCD_E2023_2024】;

基金:甘肃省重点人才项目(中医男科人才团队建设及男性性功能障碍中医诊疗技术推广应用);甘肃省中医药管理局基金项目(GZKP-2021-18);甘肃省中医药防治慢性病重点实验室开放基金(GSMBKY2015-13);甘肃中医药大学附属医院院内课题(gzfy-2019-07);兰州大学第一医院院内基金(ldyyyn2022-70);甘肃省自然科学基金(23JRRA1198)。

语种:中文

中文关键词:地龙蛋白;糖尿病;勃起功能障碍;RhoA/Rho;大鼠

外文关键词:earthworm protein;diabetes mellitus;erectile dysfunction;RhoA/Rho;rats

摘要:目的探讨地龙蛋白对糖尿病勃起功能障碍(DMED)大鼠阴茎海绵体RhoA/Rho信号通路的影响,明确地龙蛋白治疗DMED的作用机制。方法将60只SD大鼠随机选取10只为空白组,余下50只通过注射链脲佐菌素(STZ)构建糖尿病大鼠模型,然后用阿朴吗啡(APO)筛选符合DMED大鼠模型。将DMED大鼠随机分为模型组,西药组,地龙蛋白低、中、高剂量组。空白组、模型病组给予生理盐水灌胃,西药组予以西地那非灌胃,地龙蛋白低、中、高剂量组分别予以地龙蛋白灌胃。4周后测定各组大鼠阴茎海绵体最大内压(Max ICP)/平均颈动脉压(MAP)的比值。使用硝酸还原酶法检测大鼠血清中NO水平,ELISA法检测大鼠血清中ET水平,免疫组化、免疫荧光分别检测大鼠阴茎组织中RHOA、eNOS的表达,Masson染色观察阴茎组织结构的改变,并采用Real-time PCR检测阴茎海绵体组织中ROCK1、ROCK2的mRNA表达,采用Western blot的方法检测阴茎海绵体组织中ROCK1、ROCK2、P-MYPT1的蛋白表达。结果与空白组相比,模型组大鼠勃起功能明显下降(P<0.01);血清NO、阴茎组织eNOS表达明显下降(P<0.01);血清ET水平,阴茎组织RhoA、ROCK1、ROCK2、P-MYPT1表达显著升高(P<0.01);与模型组相比,各治疗组大鼠阴茎勃起功能显著改善(P<0.01);血清NO、阴茎组织eNOS表达明显增加(P<0.05、P<0.01);血清ET水平,阴茎组织RhoA、ROCK1、ROCK2、P-MYPT1表达明显下降(P<0.05、P<0.01)。结论地龙蛋白可能通过抑制RhoA/Rho信号通路,改善eNOS、NO水平,从而来改善DMED大鼠的勃起功能。
Objective To investigate the effect of earthworm protein on RhoA/Rho signal pathway in penile corpus cavernosum of diabetes mellitus erectile dysfunction(DMED)rats,and clarify its mechanism in the treatment of DMED.Methods Among 60 SD rats,10 rats were randomly selected as the blank group,and the other 50 rats were used to establish diabetic rat models by injecting with streptozotocin(STZ),and then DMED rat models were screened by apomorphine(APO)test.DMED rat models were further divided into five groups such as the model group,western medicine group,low,middle and high dose earthworm protein groups.The rats in western medicine group were given sildenafil,the rats in the blank group and the model group were given normal saline,and the rats in low,middle and high dose groups of earthworm protein were given earthworm protein.The maximum internal pressure(Max ICP)/average carotid artery pressure(MAP)of penis cavernous body was measured after 4 weeks.The level of serum NO in the rats was detected by nitrate reductase method,the level of serum ET in the rats was detected by ELISA,the expressions of RhoA and eNOS in rat penis tissue were detected by immunohistochemistry and immunofluorescence,the changes of penis tissue structure were observed by MASSON staining,the mRNA expressions of ROCKI and ROCK2 in penis cavernous tissue were detected by Real-timePCR,and the protein expressions of ROCKI,ROCK2 and P-MYPTI in penis cavernous tissue were detected by Western blot.Results Compared with that in the blank group,the erectile function of rats in the model group was significantly decreased(P<0.01),and their serum NO level and eNOS expression in penis tissue were also remarkably decreased(P

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