文献类型：期刊文献

英文题名：Hydroxytyrosol Alleviated Hypoxia-Mediated PC12 Cell Damage through Activating PI3K/AKT/mTOR-HIF-1 α Signaling

作者：Li, Xiaolin[1,2,3]; Tian, Xiuyu[1,2,4]; Liu, Tianlong[1,2]; Li, Maoxing[1,2,3,4,5]; Wang, Weigang[1,2,3]; Wang, Peng[1,2,3]; Guo, Ziliang[1,2,4]

第一作者：Li, Xiaolin;李小林

机构：[1] Department of Clinical Pharmacy, The 940th Hospital of Joint Logistic Support Force of Chinese People's Liberation Army, Lanzhou, 730050, China; [2] Gansu Plateau Pharmaceutical Technology Center, Lanzhou, 730050, China; [3] College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou, 730000, China; [4] College of Pharmacy, Lanzhou University, Lanzhou, 730000, China; [5] Institute of Chemical Technology, Northwest Minzu University, Lanzhou, 730030, China

第一机构：Department of Clinical Pharmacy, The 940th Hospital of Joint Logistic Support Force of Chinese People's Liberation Army, Lanzhou, 730050, China

通信机构：[1]Department of Clinical Pharmacy, The 940th Hospital of Joint Logistic Support Force of Chinese People's Liberation Army, Lanzhou, 730050, China

年份：2022

卷号：2022

外文期刊名：Oxidative Medicine and Cellular Longevity

收录：EI(收录号：20222512261095);Scopus(收录号：2-s2.0-85131850747)

语种：英文

外文关键词：Antioxidants - Cell signaling - Enzyme activity - Oxygen - Signaling

摘要：Background. Hypoxia exerts pressure on cells and organisms, and this pressure can occur under both pathological and nonpathological conditions. There are many reports confirmed that hydroxytyrosol has good in vitro antioxidant activity, while the research about hydroxytyrosol on hypoxia-mediated cell damage is still unclear. Purpose. The aim of this study was to investigate the effect and mechanism of hydroxytyrosol on hypoxia-mediated cell damage. Methods. We studied the effects of hydroxytyrosol on the content of reactive oxygen species, the change of antioxidant enzymes activity of SOD, CAT, and GSH-Px and the content of oxidation products MDA and GSH, and the changes of cell membrane potential and effect on PI3K/AKT/mTOR-HIF-1α signaling pathway under hypoxia-mediated PC12 cell. Results. PC12 cell treated with hydroxytyrosol abated the cell apoptosis and alleviated the oxidative stress through scavenging of reactive oxygen species, improving the enzyme activity and changing the content of oxidation products and alleviating mitochondria damage. Western blotting confirmed that the mechanism maybe related to the PI3K/AKT/mTOR-HIF-1α signaling pathway. The inhibition experiment confirmed that hydroxytyrosol takes part in the expression of protein PI3K and p-mTOR. Conclusion. Hydroxytyrosol reduced the oxidative stress and resisted the inhibition of PI3K/AKT/mTOR-HIF-1α signaling pathway caused by hypoxia, improved cell apoptosis, and ameliorated the antihypoxia ability of PC12 cells under hypoxia. ? 2022 Xiaolin Li et al.