文献类型：期刊文献

中文题名：基于MAPK/ERK通路探讨补肾助排汤改善多囊卵巢综合征大鼠胰岛素抵抗的机制研究

英文题名：Effects of Bushen Zhupai Decoction(补肾助排汤) on insulin resistance in MAPK/ERK signa-ling pathway and biochemical indexes of rats with polycystic ovary syndrome

作者：张小花[1,2];蔺文娟[1];武权生[1,2];张舒媛[1];李鹤[1];万文文[1];袁荣荣[1];芮守月[1]

第一作者：张小花

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃中医药大学附属医院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2025

卷号：36

期号：11

起止页码：2048

中文期刊名：时珍国医国药

外文期刊名：Lishizhen Medicine and Materia Medica Research

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金项目(82260949);甘肃省教育厅创新基金项目(2022A-065);甘肃省中医药管理局科研课题(GZKP-2023-44);甘肃省中医药研究中心开放课题(zyzx-2023-13)。

语种：中文

中文关键词：多囊卵巢综合征;胰岛素抵抗;MAPK/ERK信号通路;补肾助排汤

外文关键词：Polycystic ovary syndrome;Insulin resistance;MAPK/ERK signaling pathway;Bushen Zhupai Decoction(补肾助排汤)

摘要：目的 研究补肾助排汤对多囊卵巢综合征(PCOS)大鼠胰岛素抵抗(IR)的改善作用,及对MAPK/ERK信号通路、生化指标的影响。方法 成功构建大鼠模型后随机分组,各组治疗28d,记录大鼠体重变化,HE染色观察卵巢组织,油红O染色观察肝脏脂肪变化,生化仪测定空腹血糖(FBG)等生化指标,ELISA测定血清胰高血糖素样肽1(GLP-1)、空腹胰岛素(FINS),WB检测卵巢组织MAPK/ERK通路相关蛋白表达,qPCR方法检测MEK、ERK、P38MAPK mRNA的表达。结果 与空白组相比,模型组大鼠体质量、GLP-1、FINS、FBG等生化指标升高(P<0.01),卵巢呈多囊样改变,肝脏脂滴积聚,卵巢组织中P-MEK、P-ERK蛋白表达升高(P<0.01),MEK、ERK、P38MAPK mRNA水平升高(P<0.01);与模型组相比,各治疗组大鼠卵巢多囊样变改善,肝脏脂滴减少,体质量减轻,GLP-1、FINS、FBG等生化指标降低,卵巢组织中MEK、ERK、P38MAPK mRNA及P-MEK、P-ERK蛋白表达降低,以中药高剂量组明显(P<0.01)。结论 补肾助排汤可以改善大鼠PCOS-IR状态,该作用可能与抑制MAPK/ERK信号通路有关。
Objective To study the effect of Bushen Zhupai Decoction(补肾助排汤,BSZPD)on relieving insulin resistance(IR)in rats with polycystic ovary syndrome(PCOS),and on MAPK/ERK signaling pathway and biochemical indexes.Methods The rat model was successfully constructed and randomly divided into four groups.After treatment for 28 days,the weight change of rats was recorded,ovarian tissue observed by HE staining,liver fat change observed by oil red O staining,fasting blood glucose(FBC)and other biochemical indexes determined by biochemical analyzer,serum glucagon-like peptide 1(CLP-1)and fasting insulin(FINS)determined by ELISA.The expression of MAPK/ERK pathway-related proteins in ovarian tissue was detected by Western blot(WB),and the mRNA expression of MEK,ERK and P38MAPK was detected by qPCR.Results Compared with the blank group,body mass(BM),GLP-1,FINS,FBG and other biochemical indexes of rats in the model group were increased(P<0.01),ovary showed polycystic change,liver lipid droplets accumulated,and the expression of P-MEK and P-ERK protein in ovarian tissue was increased(P