文献类型：期刊文献

英文题名：The Codonopsis pilosula water extract improves testicular inflammatory aging in D-galactose induced aging mice by modulating the CLEC7A/ inflammasome pathway

作者：Wang, Jing[1];Li, Xuechan[1];Li, Caihong[1];Liu, Lijun[2];Wang, Zhenjuan[1];Feng, Juan[3]

第一作者：王婧;王晶;王静

通信作者：Feng, J[1]

机构：[1]Gansu Univ Chinese Med, Sch Publ Hlth, Lanzhou 730000, Peoples R China;[2]Lanzhou Univ, Clin Med Coll 1, Lanzhou 730000, Peoples R China;[3]Shenzhen Technol Univ, Coll Hlth Sci & Environm Engn, 3002 Lantian Rd, Shenzhen 518118, Peoples R China

第一机构：甘肃中医药大学公共卫生学院

通信机构：[1]corresponding author), Shenzhen Technol Univ, Coll Hlth Sci & Environm Engn, 3002 Lantian Rd, Shenzhen 518118, Peoples R China.

年份：2025

卷号：346

外文期刊名：JOURNAL OF ETHNOPHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-105000463728);WOS:【SCI-EXPANDED(收录号:WOS:001456061900001)】；

基金：Funding statement This work was supported by the National Natural Science Foundation of China (No.82060829 and No.82204759) , the Natural Sciences

语种：英文

外文关键词：Aging; Testicular inflammation; Codonopsis pilosula; D -galactose; CLEC7A/Inflammasome pathway

摘要：Aim of the study: Aging-induced testicular inflammation impairs male fertility. The purpose of this study was to investigate the effectiveness and mechanism of C. pilosula water extract (CPWE) in preventing testicular inflammation in D-galactose-induced aging mice. Materials and methods: The "The Plant List" database (www.theplantlist.org) provided verified plant taxonomy. D-galactose was intraperitoneally injected to induce an aging mice model, with high, medium, and low dosages of CPWE used as pharmacological interventions. The concentrations of superoxide dismutase (SOD), malondialdehyde (MDA), testosterone and in mouse serum or testicle samples after CPWE treatment were quantified using biochemical method. Hematoxylin and eosin (HE) staining was employed to assess the morphological features of testicular tissues, whereas immunohistochemical (IHC) analysis and enzyme-linked immunosorbent assay (ELISA) were conducted to evaluate the presence and levels of inflammatory cytokines interleukin-6 (IL-6) and interleukin-1R (IL-1R) within testicular samples of mice. Differentially expressed genes were identified using transcriptome sequencing; the genes and pathways regulated by CPWE, as well as immune cell infiltration, were examined using bioinformatics analysis. The expression of target gene and pathway-related protein was confirmed using real-time quantitative PCR and Western blotting. Results: Treatment with CPWE alleviated the pathological alterations in the testicular tissues of aged mice, increased the concentrations of SOD and testosterone in the serum, and decreased the levels of MDA, IL-6 and IL1R in the testes. The expression of C-C motif chemokine ligand 21a (Ccl21a) and C-C motif chemokine ligand 27b (Ccl27b) genes was downregulated after treatment with CPWE. The protein levels associated with the C-type lectin domain family 7, member A (CLEC7A)/inflammasome signaling pathway, including IL-1R, Caspase 8 (CASP8), and nuclear factor-kappa B (NF-kappa B), were found to be downregulated after treatment with CPWE. T cells, B cells, and macrophages showed a strong association with aging and the modulatory effects of CPWE. Conclusions: The results indicate that CPWE regulates the CLEC7A/inflammasome pathway, thereby inhibiting inflammasomes activation and reducing the expressions of proinflammatory cytokines such as IL-6 and IL-1R, as well as chemokines such as Ccl21a and Ccl27b, providing substantial protection against age-related testicular inflammatory injury.