文献类型：期刊文献

中文题名：旱盐双重胁迫对乌拉尔甘草幼苗生理生化特性的影响

英文题名：Effect of drought and salt stress on the physiological and biochemical characteristics of Glycyrrhiza uralensis

作者：耿广琴[1];谢晓蓉[1]

第一作者：耿广琴

机构：[1]甘肃中医药大学基础医学院

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2018

卷号：35

期号：9

起止页码：2166

中文期刊名：草业科学

外文期刊名：Pratacultural Science

收录：CSTPCD;;CSCD:【CSCD2017_2018】；

基金：甘肃省自然科学基金项目"甘草在旱盐交叉胁迫下的适应机理研究"(1506RJZA047)

语种：中文

中文关键词：逆境胁迫;生长量;渗透调节物质;抗氧化;DNA损伤;生理防御

外文关键词：adversity stress;growth;osmotic adjustment substance;antioxidant;DNA damage;physiological defense

摘要：以一年生乌拉尔甘草(Glycyrrhiza uralensis)为材料,采用盆栽试验研究旱盐双重胁迫对乌拉尔甘草生长量,可溶性糖和脯氨酸含量,超氧化物歧化酶(SOD)和丙二醛(MDA)含量,以及原生质体DNA的影响。结果显示,在旱盐双重胁迫下,乌拉尔甘草株高、鲜重、干重下降。在NaCl浓度小于400mmol·L-1时,乌拉尔甘草生长量在轻度与中度干旱处理间无显著差异(P>0.05)。轻度、中度干旱胁迫下,随盐分含量增加,甘草幼苗脯氨酸含量和可溶性糖含量明显升高,幼苗叶片SOD活力与MDA含量持续上升,幼苗原生质体DNA尾长、尾矩、Olive尾矩持续升高(P<0.05)。测定结果表明,乌拉尔甘草具有适应一定程度旱盐双重胁迫的能力,其适宜的胁迫条件为NaCl浓度小于400mmol·L-1,中度干旱胁迫。与干旱相比,盐分是影响甘草生长量的主导因子。
A pot experiment was conducted to study the influence of drought and salt stress on the growth;proline,soluble sugar,and malondialdehyde(MDA)content;superoxide dismutase(SOD)activity;and protoplast DNA in Glycyrrhiza uralensis.The results showed the height,as well as fresh and dry weight,to decrease under drought and salt stress.When the salt factor was less than or equal to 400 mmol·L-1 NaCl,there was no significant difference in the growth of G.uralensis between mild and moderate drought treatments.Under mild and moderate drought stress,the content of proline and soluble sugar increased significantly along with the SOD activity and MDA content;the tail length,tail moment,and olive tail moment in protoplast DNA continued to increase with the increase in salt content(P<0.05).In conclusion,G.uralensis can adapt to drought and salt stress at salinity levels less than or equal to 400 mmol·L-1 NaCl and medium drought stress.Under mild and moderate drought stress,salinity is the dominant factor affecting the growth of G.uralensis as compared to drought.