文献类型：期刊文献

英文题名：Evaluation of the Effect and Mechanism of Sanhuang Ointment on MRSA Infection in the Skin and Soft Tissue via Network Pharmacology

作者：Pan, Haibang[1];Wang, Tianming[1,2];Che, Ying[3,4];Li, Xiaoli[1,5];Cui, Yan[1];Chen, Quanxin[1];Wu, Zhihang[1];Yi, Jianfeng[1];Wang, Bo[3]

第一作者：潘海邦

通信作者：Yi, JF[1];Wang, B[2]

机构：[1]Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou, Peoples R China;[2]Gansu Prov New Prod Tradit Chinese Med Prod Creat, Gansu Prov Key Lab Tradit Chinese Med Recipe Min &, Lanzhou, Peoples R China;[3]Gansu Univ Chinese Med, Sch Nursing, Lanzhou, Peoples R China;[4]Gansu Prov Peoples Hosp, Res Ward, Lanzhou, Peoples R China;[5]Gansu Prov Matern & Child Care Hosp, Lanzhou, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Sch Nursing, Lanzhou, Peoples R China.|[107352f23a9081f8152fc]甘肃中医药大学护理学院;[10735]甘肃中医药大学;

年份：2023

卷号：16

起止页码：7071

外文期刊名：INFECTION AND DRUG RESISTANCE

收录：;Scopus(收录号：2-s2.0-85176776590);WOS:【SCI-EXPANDED(收录号:WOS:001103041800001)】；

基金：Funding This research was supported by the National Natural Science Foundation of China (81860850) , the Gansu Provincial Department of Education Project (2021CXZX-734 and 2021CXZX-736) , and the Gansu Provincial Higher Education Innovation Project (2023S-76) .

语种：英文

外文关键词：Sanhuang ointment; MRSA; SSTI; network pharmacology; IL-17; NF-kappa B

摘要：Introduction: Skin and soft tissue infection (SSTI) is a frequently encountered clinical disease, and Sanhuang ointment, a traditional Chinese medicine, is used to treat it. However, the pharmacological effect of Sanhuang ointment on SSTI and its underlying mechanism remains unclear. Here, we investigate the protective effect of Sanhuang ointment on Methicillin-resistant Staphylococcus aureus (MRSA) infection in the skin and soft tissues and the underlying mechanism by network pharmacological analysis, followed by in vivo experimental validation.
Methods: Via network pharmacology, the active components and disease targets of Sanhuang ointment were screened and intersected for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. A rat model of skin and soft tissue infection was established, and pathological features were observed. Large, medium, and small-dose groups (1 g, 0.5 g, and 0.25 g/animal, with the total amount of Vaseline, dispensed 1 g/animal) of Sanhuang ointment were prepared and Mupirocin ointment was used as a positive control (0.5 g/animal, with the total amount of Vaseline, dispensed 1 g/animal). The expressions of key proteins of the IL-17/NF-kappa B signaling pathway and downstream inflammatory factors were analyzed by histomorphological analysis, enzyme-linked immunosorbent assay, polymerase chain reaction, and Western blotting.
Results: In all, 119 active components and 275 target genes of Sanhuang ointment were identified and intersected with MRSA infection-related genes via network pharmacology analysis, and 34 target genes of Sanhuang ointment were found to be involved in skin and soft tissue infections with MRSA. Sanhuang ointment (1 g/mouse) could effectively ameliorate histopathological changes and significantly inhibit the expression of key proteins involved in the IL-17/NF-kappa B signaling pathway and downstream inflammatory factors (p < 0.05).
Conclusion: Sanhuang ointment has a protective effect on MRSA infection and inhibits inflammation by inhibiting the IL-17/NF-kappa B signaling pathway. Our findings are important for the secondary development and new drug development of Sanhuang ointment.