文献类型：期刊文献

英文题名：Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function

作者：Wang, Chunai[1];Liang, Xi[1];Yu, Yan[1];Li, Yulan[2];Wen, Xiaohui[3];Liu, Min[4]

第一作者：Wang, Chunai

通信作者：Wang, CA[1]

机构：[1]Gansu Prov Hosp Tradit Chinese Med, 424 Guazhou Rd, Lanzhou 730050, Gansu, Peoples R China;[2]Lanzhou Univ, Hosp 1, Lanzhou, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Lanzhou, Gansu, Peoples R China;[4]Gansu Univ Chinese Med, Affiliated Hosp, Lanzhou, Gansu, Peoples R China

第一机构：Gansu Prov Hosp Tradit Chinese Med, 424 Guazhou Rd, Lanzhou 730050, Gansu, Peoples R China

通信机构：[1]corresponding author), Gansu Prov Hosp Tradit Chinese Med, 424 Guazhou Rd, Lanzhou 730050, Gansu, Peoples R China.

年份：2020

卷号：25

期号：1

外文期刊名：EUROPEAN JOURNAL OF MEDICAL RESEARCH

收录：;Scopus(收录号：2-s2.0-85088907347);WOS:【SCI-EXPANDED(收录号:WOS:000560016000001)】；

基金：This work was supported by Regional Science Fund Project (81760892)

语种：英文

外文关键词：Bupivacaine; Electroacupuncture preconditioning; Cardiac mitochondria; Neiguan point; Lipid emulsion

摘要：Background Electroacupuncture is well known for its advantageous neuroanalgesic and therapeutic effects on myocardial ischemia-reperfusion injury. The purpose of the present research was to verify whether electroacupuncture can alleviate bupivacaine-induced myocardial injury. Methods Specific pathogen-free Wistar rats were used to establish the bupivacaine-induced myocardial injury model. Western blot, PCR, transmission electron microscope and enzyme-linked immunosorbent (ELISA) methods were used to evaluate bupivacaine-induced structure injury and dysfunction of the mitochondria as well as the alleviating effects of lipid emulsion, acupoint injection, and electroacupuncture pre-treatment of the oxidase stress response. Results Bupivacaine caused structural damage, degradation, and swelling of mitochondria. Furthermore, it reduced adenosine triphosphate (ATP) synthesis and impaired energy metabolism in the mitochondria. Structural and functional impairment of the mitochondria was alleviated via lipid emulsion injection, acupoint injection, and electroacupuncture pre-treatment. Electroacupuncture pre-treatment of PC6 yielded a greater alleviating effect than others approaches. Following electroacupuncture pre-treatment of PC6 point, the number of mitochondria increased; apoptosis was reduced, enzymatic activity of cytochrome C oxidase (COX) and superoxide dismutase and expression of uncoupling protein 2, voltage-dependent anion channel 1, and Bcl 2 were upregulated and SLC25A6, MDA levels were downregulated. Additionally, our findings indicated that electroacupuncture pre-treatment of PC6 point exerted an effect on the mitochondria via the mitochondrial-transcription-factor-A/nuclear-respiratory-factor-1/proliferator-activated-receptor-gamma-coactivator-1 pathway. Conclusion The present study revealed that electroacupuncture pre-treatment of PC6 could effectively alleviate bupivacaine-induced myocardial mitochondrial damage, thereby providing a theoretical basis for clinical studies and applications of this treatment method.