文献类型：期刊文献

英文题名：Houpu Sanwu decoction alleviates rat intraperitoneal adhesion by regulating macrophage polarization and inhibiting inflammatory responses

作者：Liu, Mei[1,2];Chen, Quanxin[2];Cui, Yan[2];Wang, Tianming[2];Li, Qun[1];Wu, Zhihang[2];Li, Richeng[2];Tang, Yuan[2];Pan, Haibang[2,3]

第一作者：刘敏;Liu, Mei

通信作者：Pan, HB[1]

机构：[1]Lanzhou Univ Second Hosp, Dept Rehabil Med, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Clin Med Sch 1, Lanzhou 730000, Gansu, Peoples R China;[3]35 Dingxi East Rd, Lanzhou, Gansu, Peoples R China

第一机构：Lanzhou Univ Second Hosp, Dept Rehabil Med, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), 35 Dingxi East Rd, Lanzhou, Gansu, Peoples R China.

年份：2026

卷号：100

外文期刊名：TISSUE & CELL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001691117500001)】；

基金：This work was supported by research grants from National Natural Science Foundation of China, Regional Science Foundation Project (82560941) Gansu Provincial Science and Technology Department, Science and Technology Fund Program (24JRRA1024); Gansu Provincial Science and Technology Department, Youth Science and Technology Fund Program (21JR1RA159).

语种：英文

外文关键词：Intraperitoneal adhesion; Hopu Sanwu decoction; Macrophage polarization; SOCS/JAK2/STAT/PPAR-gamma pathway

摘要：Background : Intraperitoneal adhesion frequently occurs after abdominal surgery, substantially affecting patients' quality of life. This study evaluated the therapeutic effect of Houpu Sanwu decoction on intraperitoneal adhesion and its possible molecular mechanism. Methods : Thirty-six male Sprague Dawley rats were randomly assigned to sham, control, fluvastatin and low-, medium-, and high-concentration Hopu Sanwu decoction groups. All groups, except for the sham group, underwent abdominal adhesion modeling. Hematoxylin and eosin staining was conducted to observe the pathological changes in peritoneal tissue. Masson staining was used to observe the development of collagen fibers within the adhesive tissues. The protein levels of MCP-1 in adhesion tissues were detected using immunohistochemistry. The serum levels of IL-1, IL-6, TNF-alpha, IL-4, and IL-10 were detected using ELISA. RT-PCR and western blotting were utilized to identify the expression levels of the SOCS/JAK2/STAT/PPAR-gamma pathway. Results : The occurrence of intraperitoneal adhesion decreased in the low-, medium-, and high-concentration Hopu Sanwu decoction groups compared with the control group, with the most significant decrease observed in the medium-concentration group (P < 0.05). Following Hopu Sanwu decoction treatment, MCP-1 protein expression in adhesive tissue significantly decreased (P < 0.05). Furthermore, markers associated with M1-type macrophages, such as IL-1, IL-6, and TNF-alpha, exhibited decreased expression levels, whereas markers of M2-type macrophages, such as IL-4 and IL-10, increased post-Hopu Sanwu decoction treatment. Additionally, Hopu Sanwu decoction can activate the SOCS1/STAT6/PPAR-gamma pathway, inhibit the SOCS3/JAK2/STAT1 pathway, and promote the polarization of macrophages toward M2. Conclusions : These results suggest that Hopu Sanwu decoction is effective in alleviating intraperitoneal adhesion, possibly by inducing M2-type macrophage polarization and modulating the SOCS/JAK2/STAT/PPAR-gamma pathway.