文献类型：期刊文献

英文题名：Identifications of metabolic differences between Hedysari Radix Praeparata Cum Melle and Astragali Radix Praeparata Cum Melle for spleen-qi deficiency rats: A comparative study

作者：Li, Yuefeng[1,2,4];Zhang, Yugui[1,2];Cao, Rui[1,2];Niu, Jiangtao[1,2];Bian, Tiantian[1,2];Ma, Dingcai[1,2];Wang, Zhe[1,2];Wang, Maomao[1,2];Yan, Xingke[3,5]

第一作者：李越峰;Li, Yuefeng

通信作者：Yan, XK[1]

机构：[1]Gansu Univ Chinese Med, Pharm Coll, Lanzhou 730000, Peoples R China;[2]Key Lab Qual & Stand Tradit Chinese Med Gansu Prov, Lanzhou 730000, Peoples R China;[3]Gansu Univ Chinese Med, Coll Acupuncture Moxibust & Tuina, Lanzhou 730000, Peoples R China;[4]Gansu Univ Chinese Med, Sci Res & Expt Ctr, Lanzhou 730000, Peoples R China;[5]35 Dingxi East Rd, Lanzhou, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), 35 Dingxi East Rd, Lanzhou, Gansu, Peoples R China.

年份：2023

卷号：236

外文期刊名：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

收录：;Scopus(收录号：2-s2.0-85170038905);WOS:【SCI-EXPANDED(收录号:WOS:001075644400001)】；

基金：Acknowledgement This study was supported by the National Natural Science Founda-tion of China (No. 81960713 and 82160750) , Gansu Provincial Educa-tion Department Industrial Support Plan Project (2021CYZC-21) and Gansu Provincial Department of Science and Technology-Science and Technology Plan (Innovation Base and Talent Plan) Basic Research Innovation Group Project (21JR7RA569) .

语种：英文

外文关键词：Hedysari Radix Praeparata Cum Melle; Astragali Radix Praeparata Cum Melle; Spleen-qi deficiency; Metabolomics; Metabolic differences; Comparative study

摘要：Hedysari Radix Praeparata Cum Melle (HRPCM) and Astragali Radix Praeparata Cum Melle (ARPCM) are capable of improving spleen-qi deficiency (SQD) syndrome especially in the gastrointestinal dysfunction and decreased immunity in traditional Chinese medicine clinically. This study aims to compare and reveal the metabolic differences between HRPCM and ARPCM for SQD rats. Firstly, HRPCM (12.6 g/kg) and ARPCM (12.6 g/kg) were used to intervene SQD rats to further evaluate the effect. The results showed that HRPCM and ARPCM were able to improve the spleen pathology, increase the body weight, the rectal temperature, the spleen index, the thymus index, the levels of GAS and D-xylose in serum, and decrease the levels of IL-2, IL-6 and TNF-alpha in serum for SQD rats. Then, the studies of metabolic differences in serum and spleen were carried out using UPLC-Q-TOF-MS. The findings emphasized that HRPCM and ARPCM not only regulated metabolic profiling of serum and spleen in SQD rats, but also existed differences. HRPCM and ARPCM regulated metabolic pathways mainly including lipid metabolism, energy metabolism, amino acid metabolism, nucleotide metabolism, sugar metabolism and other types of metabolism for SQD rats. However, the metabolite profiles in SQD rats changed significantly, mainly involving abnormal glycine synthesis occurred in SQD rats. The expression trends of metabolites in HRPCM and ARPCM intervention for SQD rats were partly the same. Interestingly, there are similarities and differences in metabolic profiling between HRPCM and ARPCM for SQD rats. The differences were mainly in the synthesis of L-glutamine in amino acid metabolism.