文献类型：期刊文献

中文题名：理肺化纤方对肺纤维化CTGF和FN信号转导通路影响的研究

英文题名：Influence of Lifei Huaxian Decoction on Connective Tissue Growth Factor and Fibronectin Signal Transduction Pathway in Pulmonary Fibrosis

作者：周语平[1];郭宏亮[1]

第一作者：周语平

机构：[1]甘肃中医学院,兰州730000

第一机构：甘肃中医药大学

年份：2011

卷号：17

期号：14

起止页码：201

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2008】；

基金：甘肃省卫生厅中医药科研项目(甘科鉴定[2009]043号)

语种：中文

中文关键词：理肺化纤方;肺纤维化;结缔组织生长因子;纤维连接蛋白;信号转导通路;羟脯氨酸

外文关键词：Lifei Huaxian decoction; connective tissue growth factor; fibronectin; signal transduction pathway; pulmonary fibrosis; hydroxyproline

摘要：目的:用气管注射博莱霉素方法模拟肺纤维化模型,观察理肺化纤方对肺纤维化结缔组织生长因子(CTGF)信号转导通路和纤维连接蛋白(FN)信号转导通路的影响,探讨其防治肺纤维化的机制。方法:Wistar大鼠100只,随机分为5组:正常对照组、模型组、醋酸泼尼松片组、理肺化纤方组、醋酸泼尼松片加理肺化纤方组,每组20只。采用气管内滴注博莱霉素造模法造模,各组均于造模后第2天开始ig给药,醋酸泼尼松片组大鼠给予醋酸泼尼松片0.9 mg.kg-1 ig,理肺化纤方治疗组用理肺化纤方水煎液ig 3.78 g.kg-1,醋酸泼尼松片加理肺化纤方治疗组给醋酸泼尼松片和理肺化纤方水煎液ig,正常对照组和模型组给等容积生理盐水ig,各组动物分别于7,28 d随机处死10只,末次给药后2 h经腹主动脉放血法处死,取血,肺组织置于4%中性甲醛固定,肺组织病理切片HE染色和Masson染色观察肺泡炎和肺纤维化改变,血清羟脯氨酸(Hyp)含量测定法检测大鼠的肺纤维化程度;免疫组化方法观察CTGF,FN在肺组织中的表达。结果:与正常组比较,模型组大鼠肺泡炎及肺纤维化评分,血清Hyp含量,CTGF,FN表达均明显升高,差异具有统计学意义(P<0.05);与模型组比较,治疗组大鼠肺泡炎及肺纤维化评分,血清Hyp,CTGF,FN含量均明显降低,差异有统计学意义(P<0.05)。结论:理肺化纤方可能通过抑制大鼠肺组织CTGF,FN的表达,从而具有抗肺纤维化的作用。
Objective：To investigate the influence of Lifei Huaxian decoction（LFHX） on connective tissue growth factor（CTGF） and fibronectin（FN） signal transduction pathway in pulmonary fibrosis in rats.Method： Five randomized groups of Wistar rats were setup（n=20 each）.Then experimental pulmonary fibrosis induced by bleomycin injection through tracheal was carried in the rats on day 0;treatments were ig given on day 1 by as prednisone acetate tablets 0.9 mg·kg-1,LFHX 3.78 g·kg-1,prednisone acetate-LFHX joint treatment,and normal saline in the control group and the model group.Ten animals in each group were randomly killed on day 7 and day 28 by bleeding through the aorta.The killing was carried out 2 h after last ig administration;blood sample was collected and lung tissue（right middle lobe） was kept in 4% neutral formaldehyde.Alveolitis and pulmonary fibrosis were evaluated with pathological slides stained by HE and Masson.Pulmonary fibrosis was measured by content of serum hydroxyproline（Hyp）;CTGF,FN in lung tissue by immunohistochemistry.Result： Levels of alveolitis and pulmonary fibrosis,serum HYP,CTGF,FN of model group were significantly increased compared with those in control group（P0.05）;while the above indicators of treatment groups were significantly lower than those in model group（P0.05）.Conclusion： LFHX shows anti-pulmonary fibrosis action.It is likely related with CTGF,FN inhibition.