文献类型：期刊文献

中文题名：黄芪多糖对镉染毒大鼠肾损伤的保护作用

英文题名：Protective effects of Astragalus polysaccharides in renal injury under cadmium in rats

作者：安方玉[1];颜春鲁[1,2];刘永琦[3,2];金华[4];伍志伟[3];苏韫[3];牛彦强[5];柳鹏瑶[6]

第一作者：安方玉

机构：[1]甘肃中医药大学教学实验实训中心;[2]甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室;[3]甘肃中医药大学基础医学院;[4]甘肃中医药大学公共卫生学院;[5]甘肃中医药大学临床学院;[6]甘肃中医药大学中医临床学院

第一机构：甘肃中医药大学教学实验实训中心

年份：2018

卷号：34

期号：1

起止页码：49

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：甘肃省自然科学基金资助项目(1508RJZA046);甘肃中医药大学中青年基金资助项目(ZQ2014-9);甘肃中医药大学教学研究与教学改革基金资助项目(ZH-201606);甘肃中医药大学大学生创新基金资助项目;敦煌医学与转化省部共建教育部重点实验室开放基金资助项目(DHYX1213-004);甘肃省高等学校科研项目协同创新团队基金资助项目(2017C-14)

语种：中文

中文关键词：镉染毒;黄芪多糖;肾损伤;蛋白表达

外文关键词：cadmium exposure ; Astragalus polysaccharides ; renal injury ; protein expression

摘要：目的探讨黄芪多糖在镉致肾损伤过程中发挥的作用。方法按照随机数字表法将大鼠分为3组,每组12只:空白组、模型组、实验组。模型组和实验组均腹腔注射1.5 mg·kg^(-1)氯化镉;染毒的同时,实验组给予黄芪多糖20mg·kg^(-1),空白组和模型组灌胃等体积0.9%NaCl,干预和染毒每日1次,连续5周。染毒结束后,处死大鼠,摘取大鼠的肾组织,原子吸收分光光度法测定大鼠肾组织的镉(Cd)含量,比色分析法测定大鼠肾组织的超氧化物歧化酶(SOD)活性、乳酸脱氢酶(LDH)活性、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量,ELISA法检测大鼠血清白细胞介素-2(IL-2)含量和转化生长因子-β_1(TGF-β_1)的含量,同时免疫组化法观察肾组织Bcl-2和Bax的蛋白表达。结果空白组、模型组和实验组的IL-2含量分别为(117.38±10.89),(71.82±14.56),(102.46±13.82)pg·mL^(-1);这3组的TGF-β_1含量分别为(4.88±0.38),(9.77±0.21),(6.35±0.41)pg·mL^(-1);这3组的GSH-Px含量分别为(240.48±18.24),(159.26±21.06),(191.50±23.82)U·g^(-1);这3组的LDH含量分别为(1125.25±37.91),(2089.46±35.87),(1159.61±28.86)U·g^(-1);这3组的Cd含量分别为(0.02±0.01),(19.68±1.85),(12.99±2.11)mg·kg^(-1);这3组的SOD含量分别为(71.55±3.56),(39.30±3.36),(67.25±2.68)U·mg^(-1);这3组的MDA含量分别为(3.96±0.79),(7.47±0.78),(5.61±0.99)nmol·mg^(-1);这3组的Bcl-2灰度值分别为37.54±3.23,68.43±5.21,38.25±6.17;这3组的Bax灰度值分别为57.87±8.93,27.54±5.45,48.23±3.54。模型组与空白组比较,以上指标差异有统计学意义(均P<0.01);实验组与模型组比较,以上指标差异均有统计学意义(P<0.05或P<0.01)。结论黄芪多糖预处理对镉致大鼠肾氧化损伤存在保护作用,其发挥保护作用是通过调控Bcl-2/Bax蛋白表达实现的。
Objective To explore the role of Astragalus polysaccharides in renal tissue under cadmium treatment in rats. Methods The SPF Wistar rats were randomized divided into three groups： normal group,model group,experimental group. The normal group was intraperitoneally injected with 0. 9% saline solution, while the model group and the experimental group were intraperitoneally injected daily with cadmium chloride（ CdCl_2） in a single dose of 1. 5 mg·kg^（-1） body weight. At the same time,the experimental group was given Astragalus polysaccharide20 mg·kg^（-1） once a day for five consecutive weeks. The model group and the experimental group were given 0. 9% saline solution once a day for five consecutive weeks. After the last injection,all the rats were sacrificed by femoral artery sampling. The content of cadmium（ Cd） was measured by atomic absorption spectrophotometry. The levels of superoxide dismutase（ SOD）,malondialdehyde（ MDA）,lactate dehydrogenase（ LDH）,glautathione peroxidase（ GSH-Px） were checked by colorimetric analysis. The contents of interleukin-2（ IL-2）,transforming growth factor-β_1（ TGF-β_1） were determined by enzyme linked immunosorbent assay.The protein expressions of Bcl-2 and Bax were analyzed by immunohistochemical method. Results IL-2 1 evel in normal group,model group,experimental group were（ 117. 38 ± 10. 89）,（ 71. 82 ± 14. 56）,（ 102. 46 ± 13. 82）pg·mL^（-1）,respectively; TGF-β_1 level in above groups were（ 4. 88 ± 0. 38）,（ 9. 77 ± 0. 21）,（ 6. 35 ± 0. 41）pg·mL^（-1）,respectively; GSH-Px level in above groups were（ 240. 48 ± 18. 24）,（ 159. 26 ± 21. 06）,（ 191. 50 ± 23. 82） U·g^（-1）,respectively; LDH level in above groups were（ 1125. 25 ± 37. 91）,（ 2089. 46 ± 35. 87）,（ 1159. 61 ± 28. 86） U·g^（-1）,respectively; Cd level in above groups were（ 0. 02 ± 0. 01）,（ 19. 68 ± 1. 85）,（ 12. 99 ± 2. 11） mg·kg^（-1）,respectively; SOD level in above groups were（ 71. 55 ± 3. 56）,（ 39. 30 ± 3. 36）,（ 67. 25 ± 2. 68） U·mg^（-1）,respectively; MDA level in above groups were（ 3. 96 ± 0. 79）,（ 7. 47 ± 0. 78）,（ 5. 61 ± 0. 99） nmol·mg^（-1）,respectively; Bcl-2 gray level in above groups were 37. 54 ± 3. 23,68. 43 ± 5. 21,38. 25 ± 6. 17,respectively; Bax gray level in above groups were 57. 87 ± 8. 93,27. 54 ± 5. 45,48. 23 ± 3. 54,respectively. Compared with the normal group,all factors in the model group were significantly（ all P 0. 01）.Compared with the model group,all factors in the experimental group were significantly（ P 0. 05 or P 0. 01）.Conclusion Astragalus polysaccharides pretreatment has a certain protective effect against cadmium-induced renal damage in rats and the protection is achieved by the regulation of Bcl-2/Bax protein expression.