文献类型：期刊文献

中文题名：基于UPLC-Q-TOF/MS的归身和归尾补血机制的代谢组学初步研究

英文题名：Metabolomic Analysis of Enriching Blood Function of Angelica Sinensis Body and Tail Using UPLC-Q-TOF/MS

作者：杨秀娟[1,2];邓毅[1,2];吴国霞[1];杨延泽[1];杨志军[1,2];曼琼[1]

第一作者：杨秀娟

机构：[1]甘肃中医药大学药学院;[2]甘肃省高校中(藏)药化学与质量研究省级重点实验室,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2018

卷号：30

期号：2

起止页码：176

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然基金地区科学基金(81360633);甘肃省自然科学基金(1506RJZA044);甘肃省中管局项目(GZK-2016-25);甘肃省教育厅高等学校科研项目(2017A-056);甘肃省高校中(藏)药化学与质量研究省级重点实验室开放基金(zzy-2016-06)

语种：中文

中文关键词：归身;归尾;补血机制;代谢组学

外文关键词：Angelica sinensis body;Angelic sinensis tail;enriching blood mechanism;metabolomic

摘要：通过比较归身和归尾对溶血性血虚大鼠血清内源性代谢物的差异,探讨归身和归尾补血机制的异同。采用乙酰苯肼(APH)复制溶血性血虚大鼠模型,基于UPLC-Q-TOF/MS比较归身和归尾给药后的大鼠血清代谢指纹图谱,采用SIMCA-P14.0软件进行多变量统计分析和单变量统计分析,寻找差异代谢物。与空白对照组比,溶血性血虚模型组中琥珀酸盐、尿嘧啶、乳清酸、胞啶、尿苷、胆酸、焦谷氨酸、胞嘧啶、脱氧尿苷、3-甲基组氨酸、甲基腺苷在模型组中相对含量升高,而左旋谷酰胺、左旋色氨酸、异亮氨酸、PC(16∶0/16∶0)、脯氨酸相对含量降低,归身和归尾给药后,各代谢物出现不同程度的回调,归身组回调更接近正常组。同时,归身和归尾给药后可通过嘧啶代谢、氨基酸代谢、饱和和不饱和脂肪酸代谢及能量代谢等途径调节代谢通路。
The aim of the present study was to identify the different endogenous metabolites in hemolytic blood deficiency rats,and subsequently compare the different enriching-blood mechanisms of Angelica sinensis body and Angelica sinensis tail.Angelica sinensis body and Angelica sinensis tail were used to intervene the hemolytic blood deficiency rats induced by N-acetyl phenyl hydrazine(APH).Metabolomic fingerprints of rat serum samples were determined by ultrahigh-pressure liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-Q-TOF/MS).The data sets were then imported and subjected to multivariate statistical analysis and univariate statistical analysis using SIMCA-P14.0.Compared with normal group,the levels of succinate,uracil,orotice acid,cytidine,uridine,cholic acid,pyroglutamate acid,cytosine,deoxyuridine,3-methylglycine,and methyladenosine were significantly(P<0.05)elevated in model group,while the levels of L-glutamine,l-tryptophan,isoleucine,PC(16∶0/16∶0),and L-prolinein were significantly(P<0.05)decreased,indicating the hemolytic blood deficiency model was built successfully.In addition,the abnormal contents of serum metabolites could be turned over after the treatment of Angelica sinensis body and Angelica sinensis tail,and the Angelica sinensis body had better effects.On the basis of these metabolites,it appeared that the pyrimidine,amino acid metabolism,fatty acid metabolism and energy metabolism could be regulated by Angelica sinensis body and Angelica sinensis tail.